Phytochemical Analysis by HPLC-HRESI-MS and Anti-Inflammatory Activity of Tabernaemontana catharinensis

International Journal of Molecular Sciences
José Ivan MarquesSilvana Maria Zucolotto

Abstract

Tabernaemontana catharinensis (Apocynaceae) has been popularly used by folk medicine because of its anti-inflammatory, analgesic, and antiophidic properties. This study aims to analyze the flavonoids composition of the hydroethanolic extract and of the ethyl acetate (EtOAc) and butanol (BuOH) fractions of T. catharinensis leaves, as well as to evaluate their anti-inflammatory activity using in vivo models. The phytochemical profile, determined by High-Performance Liquid Chromatography-High-Resolution Electrospray Ionization-Mass Spectrometry (HPLC-HRESI-MS), showed the presence of flavonoids mainly having an isorhamnetin nucleus. The anti-inflammatory activity was evaluated in carrageenan-induced paw edema (pre- and post-treatment) with oral administration of a T. catharinensis hydroethanolic extract (50, 100, and 150 mg/kg) and of organic fractions (50 mg/kg). The extract and fractions showed antiedematogenic activity by decreasing myeloperoxidase (MPO) production. In the zymosan-air-pouch model, the extract and fractions inhibited leukocyte migration and significantly decreased the levels of various proteins, such as MPO, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. The cytotoxicity was evaluated by the 3-(4,5-dime...Continue Reading

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Citations

Sep 25, 2019·International Journal of Molecular Sciences·Cleydlenne Costa VasconcelosMaria do Socorro de Sousa Cartágenes
Jun 3, 2020·Plants·Ilaria MarchioniLaura Pistelli
Feb 11, 2021·Plants·Clarissa Marcelle NaidooNasser Al-Suhaibani
Aug 10, 2021·Preparative Biochemistry & Biotechnology·Julia Maria de Medeiros DantasEveraldo Silvino Dos Santos

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Methods Mentioned

BETA
Thin-layer
light microscopy
ELISA
Enzyme-Linked

Software Mentioned

SigmaPlot
GraphPad Prism
Data Analysis

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