Phytoglycoprotein inhibits interleukin-1beta and interleukin-6 via p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated RAW 264.7 cells.

Naunyn-Schmiedeberg's Archives of Pharmacology
Sei-Jung Lee, Kye-Taek Lim

Abstract

This study was carried out to investigate the anti-inflammatory effects of 30-kDa glycoprotein isolated from Dioscorea batatas Decne (DBD glycoprotein), which consists of carbohydrate content (61%) and protein content (39%) on lipopolysaccharide (LPS, 2 microg/ml)-stimulated RAW 264.7 cells. We found that DBD glycoprotein (200 microg/ml) has an inhibitory effect on the production of intracellular hydrogen peroxide (H(2)O(2)), on the phosphorylation of p38 mitogen-activated protein (MAP) kinase, on the DNA binding activity of activator protein-1 (AP-1), and on c-Jun and c-Fos protein expression, respectively. In addition, DBD glycoprotein treatment markedly suppressed the interleukin (IL)-1beta, IL-6, and inducible nitric oxide synthase (iNOS) expression and the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. Interestingly, IL-1beta, IL-6, and iNOS expression was significantly attenuated by treatment with protein kinase C (PKC) inhibitor (staurosporine) as well as p38 MAP kinase inhibitor (SKF86002) in LPS-stimulated RAW 264.7 cells. On the basis of these results, we assume that DBD glycoprotein has anti-inflammatory potential, which can modulate proinflammatory signal transduction in LPS-stimulated RAW 264.7 ...Continue Reading

References

Dec 15, 1988·Nature·T Kouzarides, E Ziff
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Jan 1, 1997·Free Radical Biology & Medicine·Y J SuzukiA Sevanian
Mar 7, 2000·Current Medicinal Chemistry·V E Ooi, F Liu
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A E MedvedevS N Vogel
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M Giroux, A Descoteaux
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y W HsuW W Lin
Nov 6, 2002·Journal of Biochemistry·Shigeru Nakashima
Jan 18, 2003·Cellular and Molecular Life Sciences : CMLS·M RojkindP Greenwel
Jul 19, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Maria Chiara MaiuriRosa Carnuccio
Apr 28, 2006·Scandinavian Journal of Immunology·J SchellerS Rose-John
May 26, 2006·Biochemical Pharmacology·Geoffrey GloireJacques Piette

❮ Previous
Next ❯

Citations

Mar 20, 2012·Journal of Medicinal Food·Soyoung KimTaesun Park
Jul 19, 2012·Journal of the Science of Food and Agriculture·Wei-Yi TsaiKuo-Chih Lin
Apr 6, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hong Mei MaHiroshi Nakanishi
Jun 11, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Pham Thi Thu HuongYoung Jin Jeon
Dec 28, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Pham Thi Thu HuongYoung Jin Jeon
Jun 23, 2018·Current Protein & Peptide Science·Liang ZhangStephen Cho Wing Sze
Jun 17, 2020·Journal of Food Biochemistry·Yali WangYue Geng
Feb 13, 2021·International Journal of Biological Macromolecules·Feng LiuWen Huang
Dec 14, 2011·Journal of the American Chemical Society·Lin YuanSasa Zhu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.