Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions

Frontiers in Microbiology
Elizabeth A SavoryJeff H Chang

Abstract

Rhodococcus is a genus of Gram-positive bacteria with species that can cause growth deformations to a large number of plant species. This ability to cause disease is hypothesized to be dependent on a cluster of three gene loci on an almost 200 kb-sized linear plasmid. To reevaluate the roles of some of the genes in pathogenicity, we constructed and characterized deletion mutants of fasR and four fas genes. Findings confirmed that fasR, which encodes a putative transcriptional regulator, is necessary for pathogenesis. However, three of the fas genes, implicated in the metabolism of plant growth promoting cytokinins, are dispensable for the ability of the pathogen to cause disease. We also used long-read sequencing technology to generate high quality genome sequences for two phytopathogenic strains in which virulence genes are diverged in sequence and/or hypothesized to have recombined into the chromosome. Surprisingly, findings showed that the two strains carry extremely diverse virulence plasmids. Ortholog clustering identified only 12 genes present on all three virulence plasmids. Rhodococcus requires a small number of horizontally acquired traits to be pathogenic and the transmission of the corresponding genes, via recombinat...Continue Reading

References

Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·D KlämbtF Skoog
Mar 9, 1999·Antonie van Leeuwenhoek·M J LarkinI Nagy
Jan 17, 2004·The Journal of Biological Chemistry·Hiroyuki KasaharaHitoshi Sakakibara
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N MuraiR S Hanson
Mar 1, 1968·Plant Physiology·S MatsubaraF Skoog
Jul 3, 2007·Molecular Plant-microbe Interactions : MPMI·Madhumita V Joshi, Rosemary Loria
Jan 25, 2008·Development·Christophe Anjard, William F Loomis
Jan 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ine PertryDanny Vereecke
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nathanaël DelmotteJulia A Vorholt
Aug 7, 2010·Molecular Plant-microbe Interactions : MPMI·Ine PertryDanny Vereecke
Oct 14, 2010·PLoS Genetics·Michal LetekJosé A Vázquez-Boland
Apr 19, 2011·Annual Review of Phytopathology·Elisabeth StesDanny Vereecke
Nov 22, 2011·Accounts of Chemical Research·Qi ZhangWen Liu
Apr 10, 2012·Molecular Plant-microbe Interactions : MPMI·Isolde FrancisDanny Vereecke
Aug 4, 2012·Nature·Derek S LundbergJeffery L Dangl
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 17, 2012·Nature Reviews. Microbiology·Julia A Vorholt
Dec 14, 2012·Molecular Plant-microbe Interactions : MPMI·Chang-Jie JiangHiroshi Takatsuji
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Oct 8, 2013·Applied and Environmental Microbiology·Bruno Contreras-Moreira, Pablo Vinuesa
Jan 28, 2014·The Arabidopsis Book·Joseph J Kieber, G Eric Schaller
Mar 20, 2014·Bioinformatics·Torsten Seemann
Apr 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sandra ReuterNicholas R Thomson
Jun 7, 2014·Marine Drugs·Lynsey MacintyreRuAngelie Edrada-Ebel
Nov 6, 2014·Molecular Biology and Evolution·Lam-Tung NguyenBui Quang Minh
Mar 11, 2015·Environmental Microbiology·Janine HinschPaul Tudzynski
May 23, 2015·Genome Announcements·Elham EhsaniRamiro Vilchez-Vargas
Jun 24, 2015·Bioinformatics·Ryan R WickKathryn E Holt
Aug 8, 2015·Plant Physiology·Venkatesan RadhikaHitoshi Sakakibara
Dec 4, 2015·Nature·Yang BaiPaul Schulze-Lefert
Feb 16, 2016·New Biotechnology·Isolde M FrancisDanny Vereecke
Mar 1, 2016·Nature Reviews. Microbiology·Hao Chung TheStephen Baker
Jan 13, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Bruce A McDonald, Eva H Stukenbrock

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Citations

Oct 9, 2020·Molecular Plant-microbe Interactions : MPMI·Alexandra J WeisbergChristopher R Clarke
May 26, 2021·Annual Review of Phytopathology·Alexandra J WeisbergJeff H Chang
Nov 30, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alexandra J WeisbergJeff H Chang
Sep 21, 2021·Molecular Plant-microbe Interactions : MPMI·Xing ChenLaixin Luo
Jan 25, 2022·Molecular Plant-microbe Interactions : MPMI

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

NEBuilder Assembly Tool
MinIT
MAFFT
bbduk
BBTools
qcat
TREE
BLAST
Guppy
GraphPad

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