Phytophthora Effectors Modulate Genome-wide Alternative Splicing of Host mRNAs to Reprogram Plant Immunity.

Molecular Plant
Jie HuangSuomeng Dong

Abstract

Alternative splicing (AS) of pre-mRNAs increases transcriptome and proteome diversity, regulates gene expression through multiple mechanisms, and plays important roles in plant development and stress responses. However, the prevalence of genome-wide plant AS changes during infection and the mechanisms by which pathogens modulate AS remain poorly understood. Here, we examined the global AS changes in tomato leaves infected with Phytophthora infestans, the infamous Irish famine pathogen. We show that more than 2000 genes exhibiting significant changes in AS are not differentially expressed, indicating that AS is a distinct layer of transcriptome reprogramming during plant-pathogen interactions. Furthermore, our results show that P. infestans subverts host immunity by repressing the AS of positive regulators of plant immunity and promoting the AS of susceptibility factors. To study the underlying mechanism, we established a luminescence-based AS reporter system in Nicotiana benthamiana to screen pathogen effectors modulating plant AS. We identified nine splicing regulatory effectors (SREs) from 87 P. infestans effectors. Further studies revealed that SRE3 physically binds U1-70K to manipulate the plant AS machinery and subsequentl...Continue Reading

References

Dec 14, 1999·The Journal of Biological Chemistry·M Golovkin, A S Reddy
Dec 13, 2006·Nucleic Acids Research·Eddo KimGil Ast
Feb 26, 2009·Cell·Markus C WahlReinhard Lührmann
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Feb 5, 2010·The Plant Journal : for Cell and Molecular Biology·Brendan S KelleyJocelyn K C Rose
Apr 26, 2011·Trends in Cell Biology·Joonhee HanXiang-Dong Fu
Jul 19, 2011·Wiley Interdisciplinary Reviews. RNA·Anireddy S N Reddy, Gul Shad Ali
Nov 3, 2011·Plant Physiology·Anireddy S N ReddyAndrea Barta
Apr 10, 2012·Current Opinion in Plant Biology·Tolga O BozkurtSophien Kamoun
Aug 24, 2012·The Plant Journal : for Cell and Molecular Biology·Julie ThomasAnireddy S N Reddy
Feb 5, 2013·Nature Genetics·Yongli QiaoWenbo Ma
Nov 2, 2013·The Plant Cell·Anireddy S N ReddyAndrea Barta
Mar 4, 2014·PLoS Biology·Benjamin Petre, Sophien Kamoun
Jun 12, 2014·International Journal of Molecular Sciences·Shengming YangHongyan Zhu
Sep 3, 2014·Molecular Plant Pathology·Sophien KamounFrancine Govers
Dec 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shihao ShenYi Xing
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sunghee ChoHaihong Shen
Aug 27, 2015·The Plant Journal : for Cell and Molecular Biology·Zhihao LingShuqing Xu
Apr 29, 2016·Journal of Integrative Plant Biology·Arunkumar SrinivasanVittoria Brambilla
Oct 11, 2016·Current Opinion in Microbiology·Stephen C WhissonPaul Rj Birch
Dec 14, 2017·Nature Communications·Jie HuangSuomeng Dong

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Citations

Jan 5, 2021·Frontiers in Plant Science·Zunaira Afzal NaveedGul Shad Ali
Nov 4, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kanglu ZhaoZhiyong Xiong

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