Phytophthora infestans RXLR-WY Effector AVR3a Associates with Dynamin-Related Protein 2 Required for Endocytosis of the Plant Pattern Recognition Receptor FLS2

PloS One
Angela Chaparro-GarciaS Kamoun

Abstract

Pathogens utilize effectors to suppress basal plant defense known as PTI (Pathogen-associated molecular pattern-triggered immunity). However, our knowledge of PTI suppression by filamentous plant pathogens, i.e. fungi and oomycetes, remains fragmentary. Previous work revealed that the co-receptor BAK1/SERK3 contributes to basal immunity against the potato pathogen Phytophthora infestans. Moreover BAK1/SERK3 is required for the cell death induced by P. infestans elicitin INF1, a protein with characteristics of PAMPs. The P. infestans host-translocated RXLR-WY effector AVR3a is known to supress INF1-mediated cell death by binding the plant E3 ligase CMPG1. In contrast, AVR3aKI-Y147del, a deletion mutant of the C-terminal tyrosine of AVR3a, fails to bind CMPG1 and does not suppress INF1-mediated cell death. Here, we studied the extent to which AVR3a and its variants perturb additional BAK1/SERK3-dependent PTI responses in N. benthamiana using the elicitor/receptor pair flg22/FLS2 as a model. We found that all tested variants of AVR3a suppress defense responses triggered by flg22 and reduce internalization of activated FLS2. Moreover, we discovered that AVR3a associates with the Dynamin-Related Protein 2 (DRP2), a plant GTPase impl...Continue Reading

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Citations

Jul 1, 2016·Annual Review of Phytopathology·Tania Y ToruñoGitta Coaker
Sep 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·Viktor Žárský
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Methods Mentioned

BETA
GTPase
transgenic
immunoprecipitation
co-immunoprecipitation
confocal microscopy
PCR
GTPases
Genome

Software Mentioned

TBLASTN
RAxML
ClustalW
MUSCLE
R
Adobe Photoshop
GAMMA
Leica LAS AF
ImageJ
Mascot

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