Phytoremediation of mercury and organomercurials in chloroplast transgenic plants: enhanced root uptake, translocation to shoots, and volatilization.

Environmental Science & Technology
Hussein S HusseinHenry Daniell

Abstract

Transgenic tobacco plants engineered with bacterial merA and merB genes via the chloroplast genome were investigated to study the uptake, translocation of different forms of mercury (Hg) from roots to shoots, and their volatilization. Untransformed plants, regardless of the form of Hg supplied, reached a saturation point at 200 microM of phenylmercuric acetate (PMA) or HgCl2, accumulating Hg concentrations up to 500 microg g(-1) with significant reduction in growth. In contrast, chloroplast transgenic lines continued to grow well with Hg concentrations in root tissues up to 2000 microg g(-1). Chloroplasttransgenic lines accumulated both the organic and inorganic Hg forms to levels surpassing the concentrations found in the soil. The organic-Hg form was absorbed and translocated more efficiently than the inorganic-Hg form in transgenic lines, whereas no such difference was observed in untransformed plants. Chloroplast-transgenic lines showed about 100-fold increase in the efficiency of Hg accumulation in shoots compared to untransformed plants. This is the first report of such high levels of Hg accumulation in green leaves or tissues. Transgenic plants attained a maximum rate of elemental-Hg volatilization in two days when suppl...Continue Reading

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Citations

Mar 26, 2011·Plant Molecular Biology·Jihong Liu Clarke, Henry Daniell
Dec 21, 2010·International Journal of Phytoremediation·Jonathan M WillisMark W Hester
Nov 4, 2011·International Journal of Phytoremediation·Elena Maestri, Nelson Marmiroli
Dec 7, 2007·Plant Physiology·Dheeraj Verma, Henry Daniell
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Mar 31, 2009·Current Opinion in Biotechnology·Oscar N Ruiz, Henry Daniell
Nov 1, 2013·Environmental Toxicology and Chemistry·Liwei CuiHeng Wang
May 15, 2012·Journal of Hazardous Materials·Jianxu WangLihai Shang
Feb 19, 2015·Applied Biochemistry and Biotechnology·S ChangH Shu
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Jul 15, 2020·International Journal of Molecular Sciences·Yihe YuChoun-Sea Lin
Apr 4, 2021·International Journal of Environmental Research and Public Health·Emanuela D TiodarDorina Podar

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