PI-3-kinase and MAPK regulate mesangial cell proliferation and migration in response to PDGF

The American Journal of Physiology
G G ChoudhuryH E Abboud

Abstract

Proliferation and migration are important biological responses of mesangial cells to injury. Platelet-derived growth factor (PDGF) is a prime candidate to mediate these responses in glomerular disease. PDGF and its receptor (PDGFR) are upregulated in the mesangium during glomerular injury. We have recently shown that PDGF activates phosphatidylinositol 3-kinase (PI-3-kinase) in cultured mesangial cells. The role of this enzyme and other more distal signaling pathways in regulating migration and proliferation of mesangial cells has not yet been addressed. In this study, we used two inhibitors of PI-3-kinase, wortmannin (WMN) and LY-294002, to investigate the role of this enzyme in these processes. Pretreatment of mesangial cells with WMN and LY-294002 dose-dependently inhibited PDGF-induced PI-3-kinase activity assayed in antiphosphotyrosine immunoprecipitates. WMN pretreatment also inhibited the PI-3-kinase activity associated with anti-PDGFR beta immunoprecipitates prepared from mesangial cells treated with PDGF. Pretreatment of the cells with different concentrations of WMN resulted in a dose-dependent inhibition of PDGF-induced DNA synthesis. Both WMN and LY-294002 inhibited PDGF-stimulated migration of mesangial cells in a ...Continue Reading

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Citations

Feb 11, 2005·American Journal of Physiology. Renal Physiology·Lenin MahimainathanGoutam Ghosh Choudhury
Aug 22, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Edgar A JaimesLeopoldo Raij
Jun 17, 2009·Molecular and Cellular Biology·Kenji KawadaVijay Yajnik
Sep 21, 2000·American Journal of Physiology. Renal Physiology·W TianD M Cohen
Mar 14, 2008·American Journal of Physiology. Renal Physiology·Damien D PearseEdgar A Jaimes
Apr 18, 2014·American Journal of Physiology. Cell Physiology·Amit BeraGoutam Ghosh Choudhury
Apr 21, 2017·American Journal of Physiology. Renal Physiology·Falguni DasGoutam Ghosh Choudhury
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Eric CellierJean-Pierre Girolami
Jul 5, 2003·American Journal of Physiology. Renal Physiology·Yves GorinHanna E Abboud
May 16, 2001·American Journal of Physiology. Renal Physiology·P M GhoshJ I Kreisberg
Mar 24, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jennifer Gillibert-DuplantierJean Rosenbaum
Jun 24, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Balachandar VenkatesanGoutam Ghosh Choudhury
Jan 15, 2002·American Journal of Physiology. Renal Physiology·Jill M RiconoHanna E Abboud
Jun 19, 2009·American Journal of Physiology. Renal Physiology·Balakuntalam S KasinathMeenalakshmi M Mariappan
Jan 10, 2018·Frontiers in Pharmacology·Vasanti SuvarnaPreeti Sangave
Feb 8, 2002·American Journal of Physiology. Renal Physiology·Misako HayamaTakashi Sato
Feb 13, 2003·American Journal of Physiology. Renal Physiology·Sule SengulVecihi Batuman
Aug 31, 2001·Biochemical and Biophysical Research Communications·G Ghosh ChoudhuryH E Abboud
May 18, 1999·Biochemical and Biophysical Research Communications·G Ghosh ChoudhuryH E Abboud

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