PI3-kinase promotes TRPV2 activity independently of channel translocation to the plasma membrane

Cell Calcium
Aubin PennaFrançois-A Rassendren

Abstract

Cellular or chemical activators for most transient receptor potential channels of the vanilloid subfamily (TRPV) have been identified in recent years. A remarkable exception to this is TRPV2, for which cellular events leading to channel activation are still a matter of debate. Diverse stimuli such as extreme heat or phosphatidylinositol-3 kinase (PI3-kinase) regulated membrane insertion have been shown to promote TRPV2 channel activity. However, some of these results have proved difficult to reproduce and may underlie different gating mechanisms depending on the cell type in which TRPV2 channels are expressed. Here, we show that expression of recombinant TRPV2 can induce cytotoxicity that is directly related to channel activity since it can be prevented by introducing a charge substitution in the pore-forming domain of the channel, or by reducing extracellular calcium. In stably transfected cells, TRPV2 expression results in an outwardly rectifying current that can be recorded at all potentials, and in an increase of resting intracellular calcium concentration that can be partly prevented by serum starvation. Using cytotoxicity as a read-out of channel activity and direct measurements of cell surface expression of TRPV2, we sho...Continue Reading

References

Nov 14, 1997·Physiological Reviews·A B Parekh, R Penner
Apr 16, 1999·Nature Neuroscience·C VirginioA Surprenant
Aug 10, 2000·The Journal of Biological Chemistry·C García-MartínezA Ferrer-Montiel
Jun 5, 2001·Nature Reviews. Neuroscience·D E ClaphamC Strübing
Dec 1, 2001·Immunity·A MacKenzieA Surprenant
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·C Montell
Apr 5, 2002·Trends in Pharmacological Sciences·Martin J GunthorpeJohn B Davis
Feb 25, 2003·Advances in Experimental Medicine and Biology·Baruch Minke
May 27, 2003·Cell Calcium·Thomas Voets, Bernd Nilius
Jun 11, 2003·The Journal of Cell Biology·Yuko IwataMunekazu Shigekawa
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Jul 23, 2003·Trends in Pharmacological Sciences·Matthias P WymannMuriel Laffargue
Sep 27, 2003·Circulation Research·Katsuhiko MurakiYuji Imaizumi
Nov 20, 2003·The Journal of Biological Chemistry·Jürgen Rettinger, Günther Schmalzing
Jan 7, 2004·American Journal of Physiology. Cell Physiology·Bernd NiliusThomas Voets
Jan 9, 2004·Cell Cycle·Matthew P Stokes, W Matthew Michael
May 6, 2004·The Journal of Biological Chemistry·Séverine ChaumontFrancois Rassendren
Jun 11, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nuria García-SanzAntonio Ferrer-Montiel
Jun 15, 2004·The Journal of Biological Chemistry·Hong-Zhen HuMichael X Zhu
Jul 20, 2004·Nature Cell Biology·Vassilios J BezzeridesDavid E Clapham

❮ Previous
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Citations

Sep 23, 2011·Cardiovascular Toxicology·Nathan RobbinsJack Rubinstein
Feb 2, 2010·Nature Immunology·Tiffany M LinkMichael J Caterina
Jun 21, 2007·Annual Review of Biochemistry·Kartik Venkatachalam, Craig Montell
May 14, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Laura DwyerSang Don Koh
May 31, 2014·Nature Communications·Yuki KatanosakaKeiji Naruse
Dec 10, 2008·Expert Opinion on Therapeutic Targets·Lisa M BroadDavid Bleakman
Jul 28, 2012·Expert Opinion on Therapeutic Patents·Antonio Ferrer-MontielRosario González-Muñiz
Sep 1, 2010·Expert Review of Clinical Pharmacology·Laura VayPeter A McNaughton
Sep 27, 2014·Membranes·Clotilde Ferrandiz-HuertasAntonio Ferrer-Montiel
Feb 21, 2016·Pharmacology & Therapeutics·Susumu OhyaKatsuhiko Muraki
Jan 19, 2016·Nature Structural & Molecular Biology·Lejla ZubcevicSeok-Yong Lee
Jul 4, 2012·Experimental Neurology·Thekkethil Prashant NedungadiJ Thomas Cunningham
Mar 23, 2011·Pharmacology & Therapeutics·Katharine Helen BannerChris Poll
Dec 23, 2006·Seminars in Cell & Developmental Biology·Jiehong HuangPeter A McNaughton
Apr 26, 2013·The FEBS Journal·Alex Perálvarez-MarínRachelle Gaudet
Jul 30, 2011·British Journal of Pharmacology·Laura VayPeter A McNaughton
Apr 12, 2012·International Journal of Cancer. Journal International Du Cancer·Maria Beatrice MorelliGiorgio Santoni
May 10, 2015·Biochemical and Biophysical Research Communications·Pau Doñate-MacianAlex Perálvarez-Marín
Jun 15, 2015·Biophysical Chemistry·Kristyna BousovaJan Teisinger
Mar 27, 2009·Biochimica Et Biophysica Acta·Michaël MonetNatalia Prevarsakaya
Feb 24, 2007·Molecular Cell·Young KwonCraig Montell
Feb 27, 2007·Biochimica Et Biophysica Acta·Jon D Levine, Nicole Alessandri-Haber
Dec 17, 2016·Pharmaceuticals·Michael J Caterina, Zixuan Pang
Dec 18, 2012·Journal of Cardiovascular Pharmacology and Therapeutics·Sheryl E KochJack Rubinstein
Jan 1, 2011·Pharmaceuticals·Asia Fernández-CarvajalAntonio Ferrer-Montiel
Jan 19, 2019·International Journal of Molecular Sciences·Justyna B StartekVictor Meseguer
Jan 17, 2019·American Journal of Physiology. Cell Physiology·Toshiaki SawataniTomohisa Ishikawa
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Dec 7, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Michal Entin-Meer, Gad Keren
Mar 20, 2009·Molecular Pharmacology·Joris VriensBernd Nilius
Oct 1, 2019·ELife·Ruth A PumroyVera Y Moiseenkova-Bell
May 19, 2017·European Biophysics Journal : EBJ·Olivier MignenChristophe Vandier

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