PI4K-beta and MKNK1 are regulators of hepatitis C virus IRES-dependent translation

Scientific Reports
Joachim LupbergerChristian Thoma

Abstract

Cellular translation is down-regulated by host antiviral responses. Picornaviridae and Flaviviridae including hepatitis C virus (HCV) evade this process using internal ribosomal entry sequences (IRESs). Although HCV IRES translation is a prerequisite for HCV replication, only few host factors critical for IRES activity are known and the global regulator network remains largely unknown. Since signal transduction is an import regulator of viral infections and the host antiviral response we combined a functional RNAi screen targeting the human signaling network with a HCV IRES-specific reporter mRNA assay. We demonstrate that the HCV host cell cofactors PI4K and MKNK1 are positive regulators of HCV IRES translation representing a novel pathway with a functional relevance for the HCV life cycle and IRES-mediated translation of viral RNA.

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Citations

May 10, 2017·Nature Communications·Gholamreza HaqshenasChristian Doerig
Apr 2, 2020·International Journal of Molecular Sciences·Michael Niepmann, Gesche K Gerresheim
Oct 4, 2018·Viruses·Alessia VirzìJoachim Lupberger
May 3, 2020·International Journal of Molecular Sciences·Kaku GotoJoachim Lupberger
Aug 10, 2021·World Journal of Gastroenterology : WJG·Hui-Chun LiShih-Yen Lo

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Methods Mentioned

BETA
transfection
Assay
transfections

Software Mentioned

CellHTS2
GUItars

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