Pias3 is necessary for dorso-ventral patterning and visual response of retinal cones but is not required for rod photoreceptor differentiation

Biology Open
Christie K CamplaAnand Swaroop

Abstract

Protein inhibitor of activated Stat 3 (Pias3) is implicated in guiding specification of rod and cone photoreceptors through post-translational modification of key retinal transcription factors. To investigate its role during retinal development, we deleted exon 2-5 of the mouse Pias3 gene, which resulted in complete loss of the Pias3 protein. Pias3-/- mice did not show any overt phenotype, and retinal lamination appeared normal even at 18 months. We detected reduced photopic b-wave amplitude by electroretinography following green light stimulation of postnatal day (P)21 Pias3-/- retina, suggesting a compromised visual response of medium wavelength (M) cones. No change was evident in response of short wavelength (S) cones or rod photoreceptors until 7 months. Increased S-opsin expression in the M-cone dominant dorsal retina suggested altered distribution of cone photoreceptors. Transcriptome profiling of P21 and 18-month-old Pias3-/- retina revealed aberrant expression of a subset of photoreceptor genes. Our studies demonstrate functional redundancy in SUMOylation-associated transcriptional control mechanisms and identify a specific, though limited, role of Pias3 in modulating spatial patterning and optimal function of cone phot...Continue Reading

References

Jul 31, 2001·The Journal of Biological Chemistry·P K SwainA Swaroop
Dec 20, 2003·Cellular and Molecular Life Sciences : CMLS·D Schmidt, S Müller
Nov 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wera RothRudolf Grosschedl
Jun 16, 2005·Journal of Molecular Endocrinology·H SanttiJ J Palvimo
Jul 27, 2005·Investigative Ophthalmology & Visual Science·Melanie R RobertsThomas A Reh
Dec 6, 2005·Developmental Cell·Karim NacerddineAnne Dejean
Jan 20, 2006·The Journal of Biological Chemistry·Christoph KlenkMartin J Lohse
Mar 29, 2006·The Journal of General Physiology·Sergei S NikonovEdward N Pugh
Jul 11, 2006·Nature Reviews. Molecular Cell Biology·Bruce T SeetTony Pawson
Jul 20, 2006·Genesis : the Journal of Genetics and Development·Eric C SwindellMilan Jamrich
Jul 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Samuel TahkKe Shuai
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Ruth Geiss-Friedlander, Frauke Melchior
Nov 23, 2007·Biochemical Society Transactions·M J Lyst, I Stancheva
Dec 2, 2008·Biochemical and Biophysical Research Communications·Eun Ju HwangKeun Il Kim
Feb 3, 2009·Neuron·Akishi OnishiSeth Blackshaw
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Jun 16, 2009·Cellular and Molecular Life Sciences : CMLS·Miia M RytinkiJorma J Palvimo
Jun 17, 2010·The Journal of Biological Chemistry·Jerome E RogerAnand Swaroop
Jul 22, 2010·Nature Reviews. Neuroscience·Anand SwaroopDouglas Forrest
Aug 24, 2010·Nature Neuroscience·Akishi OnishiSeth Blackshaw
Aug 5, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lily NgDouglas Forrest
Jun 25, 2013·Progress in Retinal and Eye Research·Trevor D Lamb
Jan 3, 2014·The Journal of Clinical Investigation·Jerome E RogerAnand Swaroop
Aug 7, 2014·Nature Reviews. Neuroscience·Connie Cepko
Jan 27, 2015·Biology Open·Víctor Abad-MoralesGemma Marfany
Sep 12, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Pierre ChymkowitchJorrit M Enserink
Oct 8, 2015·Development·Joseph A Brzezinski, Thomas A Reh
Nov 14, 2015·The Journal of Biological Chemistry·Ching-Shyi Wu, Lee Zou
Sep 13, 2016·Trends in Genetics : TIG·Kayla VietsRobert J Johnston
Nov 24, 2016·Cell Reports·Jung-Woong KimAnand Swaroop

❮ Previous
Next ❯

Citations

Apr 21, 2020·Biochemical Society Transactions·Ana TalamilloRosa Barrio

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
genotyping
RNA-Seq
protein assay
dissection
chip

Software Mentioned

TopHat
Panther Classification System
PANTHER
STRING
FeatureCounts
EdgeR
GraphPad Prism
STRING ( Search Tool for the Retrieval of Interacting Genes / ...

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Edwin C T OhAnand Swaroop
Proceedings of the National Academy of Sciences of the United States of America
Melanie R RobertsThomas A Reh
© 2022 Meta ULC. All rights reserved