PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture.

Molecular Biology of the Cell
Yasuhiro Kurasawa, Li-yuan Yu-Lee

Abstract

To maintain genomic stability, chromosome architecture needs to be tightly regulated as chromosomes undergo condensation during prophase and separation during anaphase, but the mechanisms remain poorly understood. Here, we show that the Plk1-binding protein PICH and Plk1 kinase coordinately maintain chromosome architecture during prometaphase. PICH knockdown results in a loss of Plk1 from the chromosome arm and an increase in highly disorganized "wavy" chromosomes that exhibit an "open" or "X-shaped" configuration, consistent with a loss of chromosome arm cohesion. Such chromosome disorganization occurs with essentially no change in the localization of condensin or cohesin on chromosomes. Interestingly, the chromosome disorganization could be prevented by treatment with a topoisomerase II inhibitor ICRF-193, suggesting that the PICH-Plk1 complex normally maintains chromosome architecture in a manner that involves topoisomerase II activity. PICH knockdown does not affect initial chromosome compaction at prophase but causes anaphase DNA bridge formation and failed abscission. Our studies suggest that the PICH-Plk1 complex plays a critical role in maintaining prometaphase chromosome architecture.

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Citations

Apr 8, 2011·International Journal of Hematology·Songmin Ying, Ian D Hickson
Jul 4, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria Fadri-MoskwikLi-Yuan Yu-Lee
Feb 22, 2012·Journal of Cell Science·Lydia C GreenDamien F Hudson
Sep 27, 2013·PloS One·Carol ChuangLi-yuan Yu-Lee
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Jul 26, 2011·Seminars in Cell & Developmental Biology·Kok Lung Chan, Ian D Hickson
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Aug 27, 2013·Molecular Cell·Andreas BiebricherIan D Hickson
Apr 14, 2016·PloS One·Kimberly N WeiderholdLi-Yuan Yu-Lee
Aug 15, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Rongfeng LanHui Zhang
Nov 24, 2017·Cold Spring Harbor Symposia on Quantitative Biology·Kata SarlósIan D Hickson
Jul 11, 2019·Oncology Letters·Yang XieJian Qi
Dec 14, 2018·Genes·María Fernández-Casañas, Kok-Lung Chan
Jan 8, 2015·The Journal of Biological Chemistry·Vinidhra SridharanYoshiaki Azuma
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Sep 3, 2020·Molecular Biology of the Cell·Victoria A HassebroekYoshiaki Azuma
Mar 6, 2012·Journal of Cell Science·Tatiana Moutinho-SantosClaudio E Sunkel

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Methods Mentioned

BETA
immunoprecipitation
electrophoresis
transfection
Fluorescence
confocal microscopy

Software Mentioned

NIS
Elements

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