Abstract
The peroxisomal protein import machinery plays a central role in the assembly of this organelle in all eukaryotes. Genes encoding components of this machinery, termed peroxins or Pex proteins, have been isolated and characterized in several yeast species and in mammals, including humans. Here we report on one of these components, Pex14p, from the methylotrophic yeast Pichia pastoris. Work in other organisms has shown that Pex14p is located on the cytoplasmic surface of the peroxisomal membrane and binds peroxisomal targeting signal (PTS) receptors carrying proteins bound for the peroxisomal matrix, results that have led to the hypothesis that Pex14p is a receptor-docking protein. P. pastoris Pex14p (PpPex14p) behaves like an integral membrane protein, with its C-terminus exposed on the cytosolic side of the peroxisomal membrane. PpPex14p complexes with many peroxins, including Pex3p (Snyder et al., 1999b), Pex5p, Pex7p, Pex13p, Pex17p, itself, and a previously unreported peroxin, Pex8p. A portion of Pex14p is phosphorylated, but both phosphorylated and unphosphorylated forms of Pex14p interact with several peroxins. The interactions between Pex14p and other peroxins provide clues regarding the function of Pex14p in peroxisomal ...Continue Reading
References
May 20, 1992·Journal of Molecular Biology·G von Heijne
Aug 1, 1992·Journal of Bacteriology·H LiuJ M Cregg
May 24, 1991·Science·A LupasJ Stock
Mar 1, 1989·Molecular and Cellular Biology·J M CreggC A Stillman
Dec 1, 1987·The Journal of Cell Biology·S G GouldS Subramani
May 28, 1985·Biochimica Et Biophysica Acta·P KleinC DeLisi
Sep 1, 1984·Gene·I R BeachamJ Carbon
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Nov 18, 1994·Science·S FengS L Schreiber
May 5, 1995·The Journal of Biological Chemistry·H LiuJ M Cregg
May 1, 1996·Molecular and Cellular Biology·H R WaterhamJ M Cregg
Oct 1, 1996·The Journal of Cell Biology·S J GouldD I Crane
Oct 1, 1996·The Journal of Cell Biology·Y ElgersmaB Distel
Oct 1, 1996·The Journal of Cell Biology·R Erdmann, G Blobel
Dec 1, 1996·Nature Genetics·D D ShoemakerR W Davis
Jan 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H R Waterham, J M Cregg
Jan 2, 1997·The EMBO Journal·M KomoriM Veenhuis
Apr 4, 1997·Cell·M AlbertiniW H Kunau
Oct 6, 1997·The EMBO Journal·C BrocardA Hartig
Nov 15, 1997·Molecular Biology of the Cell·S E Rieder, S D Emr
Feb 21, 1998·The EMBO Journal·Y ElgersmaH F Tabak
Feb 14, 1998·The Journal of Cell Biology·B HuhseW H Kunau
Feb 3, 1998·Molecular and Cellular Biology·K N FaberS Subramani
Feb 11, 1998·Physiological Reviews·S Subramani
Mar 21, 1998·The Journal of Cell Biology·Y ElgersmaS Subramani
Jun 20, 1998·Bioinformatics·T HirokawaS Mitaku
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·M FransenS Subramani
Oct 14, 1998·Journal of Molecular Biology·G E Tusnády, I Simon
Feb 18, 1999·Molecular and Cellular Biology·G K WillR Erdmann
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·M T GeraghtyS J Gould
Mar 24, 1999·The Journal of Cell Biology·W GirzalskyR Erdmann
Apr 2, 1999·Genetics·M A JohnsonJ M Cregg
Apr 23, 1999·The Journal of Biological Chemistry·N ShimizuY Fujiki
May 5, 1999·Neurochemical Research·R J Wanders
Jun 8, 1999·Molecular Biology of the Cell·W B SnyderS Subramani
Sep 3, 1999·FEBS Letters·M KomoriM Veenhuis
Dec 10, 1999·Molecular Biology of the Cell·W B SnyderS Subramani
Jan 19, 2000·Molecular Biology of the Cell·T W BrownR A Rachubinski
Citations
Dec 2, 2005·Molecular Biology of the Cell·Lan ZhangSuresh Subramani
Jul 3, 2009·Molecular Biology of the Cell·Changle MaSuresh Subramani
Feb 18, 2012·Molecular Biology of the Cell·Saurabh JoshiSuresh Subramani
Jun 14, 2003·Plant Physiology·Cayle S LisenbeeRichard N Trelease
Oct 17, 2013·Plant Physiology·Sheng QuanJianping Hu
Mar 31, 2005·Molecular and Cellular Biology·Annette Schell-StevenRalf Erdmann
Jan 24, 2006·Molecular and Cellular Biology·Geoffrey Paul Lin-CereghinoJames M Cregg
Jan 10, 2014·Microbial Cell Factories·Katrin WeinhandlAndrea Camattari
Feb 19, 2014·The Journal of Cell Biology·Taras Y NazarkoSuresh Subramani
Oct 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya FuruyaMarilyn Parsons
Nov 13, 2004·FEMS Microbiology Reviews·Juliette MoyersoenPaul A M Michels
Feb 15, 2011·Journal of Bioscience and Bioengineering·Takashi ItoTomoyuki Nakagawa
Jul 13, 2013·FEBS Open Bio·Katsuhiro TanakaMasayuki Komori
Apr 28, 2007·Traffic·David ManagadzeHanspeter Rottensteiner
Sep 28, 2015·Biochimica Et Biophysica Acta·Michael SchraderMarkus Islinger
Oct 10, 2015·Biochimica Et Biophysica Acta·Leonidas EmmanouilidisMichael Sattler
Jan 19, 2016·Biochimica Et Biophysica Acta·Silke OeljeklausBettina Warscheid
Oct 10, 2006·Biochimica Et Biophysica Acta·Naganand Rayapuram, Suresh Subramani
Jan 6, 2005·The Journal of Biological Chemistry·Astrid KragtBen Distel
Aug 19, 2005·The Journal of Biological Chemistry·Karsten NiederhoffWolf-H Kunau
May 9, 2006·The Journal of Biological Chemistry·Wolfgang GirzalskyRalf Erdmann
Aug 23, 2008·The Journal of Biological Chemistry·Normand CyrArmando Jardim
Apr 12, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masafumi NoguchiYukio Fujiki
May 16, 2002·The Journal of Biological Chemistry·Gert Jan HaanMarten Veenhuis
May 13, 2003·Microscopy Research and Technique·Marten VeenhuisIda J Van Der Klei
Aug 25, 2020·ELife·Kanji OkumotoYukio Fujiki
Jul 13, 2014·The Journal of Biological Chemistry·Shigehiko TamuraYukio Fujiki
Mar 25, 2019·Virulence·Jiao-Yu WangGuo-Chang Sun
Jul 18, 2002·Traffic·Partha P HazraSuresh Subramani
Aug 28, 2020·The Journal of Cell Biology·Koichiro YamashitaYukio Fujiki
Oct 13, 2020·Frontiers in Cell and Developmental Biology·Andreas SchummerBettina Warscheid
Feb 23, 2002·The Journal of Biological Chemistry·Courtney C HarperStephen J Gould
Sep 11, 2002·Biochimica Et Biophysica Acta·Marten Veenhuis, Ida J van der Klei
Oct 18, 2002·Current Opinion in Cell Biology·Ida van der Klei, Marten Veenhuis
Nov 28, 2002·Molecular Cell·Alice DouangamathMatthias Wilmanns
Sep 3, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Terence InfantShirisha Nagotu
Mar 12, 2008·Developmental Cell·Jean-Claude FarréSuresh Subramani