PMID: 9546172Apr 18, 1998Paper

Pichia stipitis genes for alcohol dehydrogenase with fermentative and respiratory functions

Applied and Environmental Microbiology
J Y Cho, T W Jeffries

Abstract

Two genes coding for isozymes of alcohol dehydrogenase (ADH); designated PsADH1 and PsADH2, have been identified and isolated from Pichia stipitis CBS 6054 genomic DNA by Southern hybridization to Saccharomyces cerevisiae ADH genes, and their physiological roles have been characterized through disruption. The amino acid sequences of the PsADH1 and PsADH2 isozymes are 80.5% identical to one another and are 71.9 and 74.7% identical to the S. cerevisiae ADH1 protein. They also show a high level identity with the group I ADH proteins from Kluyveromyces lactis. The PsADH isozymes are presumably localized in the cytoplasm, as they do not possess the amino-terminal extension of mitochondrion-targeted ADHs. Gene disruption studies suggest that PsADH1 plays a major role in xylose fermentation because PsADH1 disruption results in a lower growth rate and profoundly greater accumulation of xylitol. Disruption of PsADH2 does not significantly affect ethanol production or aerobic growth on ethanol as long as PsADH1 is present. The PsADH1 and PsADH2 isozymes appear to be equivalent in the ability to convert ethanol to acetaldehyde, and either is sufficient to allow cell growth on ethanol. However, disruption of both genes blocks growth on eth...Continue Reading

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Citations

Mar 2, 2002·Applied and Environmental Microbiology·Yong-Su JinThomas W Jeffries
Mar 23, 2011·Journal of Industrial Microbiology & Biotechnology·Rita C L B RodriguesThomas W Jeffries
Jul 21, 2006·Journal of Industrial Microbiology & Biotechnology·Yong-Cheol ParkGeorge N Bennett
Jul 17, 2010·Applied Microbiology and Biotechnology·Surisa SuwannarangseeOhsuk Kwon
Jan 7, 2005·Applied Microbiology and Biotechnology·Yong-Su JinThomas W Jeffries
Aug 15, 2013·Biotechnology and Applied Biochemistry·Ievgeniia A TiukovaVolkmar Passoth
Nov 17, 2011·Applied Microbiology and Biotechnology·Tiezheng YuanBin Yao
Apr 18, 2008·Applied Biochemistry and Biotechnology·Rita C L B RodriguesThomas W Jeffries
Oct 31, 2006·Applied and Environmental Microbiology·Marja IlménMerja Penttilä
Nov 18, 2015·Biotechnology Letters·Mert KaraoglanMehmet Inan
May 29, 1999·Applied and Environmental Microbiology·J Y Cho, T W Jeffries
Jul 24, 2003·Mechanisms of Ageing and Development·Suzan J Holt, Donald L Riddle

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