Abstract
The role of oxidative stress has been well documented in the development of sepsis-induced acute lung injury (ALI). Protein interaction with C-kinase 1 (PICK1) participates in oxidative stress-related neuronal diseases. However, its function in lung infections and inflammatory diseases is not known. We therefore sought to investigate whether PICK1 is involved in sepsis-induced ALI. Cecal ligation and puncture (CLP) was performed in anesthetized wild type (WT) and PICK1 knock out (KO, PICK1-/-) mice with C57BL/6 background. At the time of CLP, mice were given fluid resuscitation. Mouse lungs were harvested at 24 and 72 h for Western blot analysis, qRT-PCR, BALF analysis, Hematoxylin and Eosin staining, TUNEL staining, maleimide staining, flow cytometry analysis, GCL, GSH, GSSG and cysteine levels measurement. A marked elevation of PICK1 mRNA and protein level were demonstrated in lung tissue, which was accompanied by increased production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) and consumption of glutathione (GSH). N-acetylcysteine (NAC), buthionine sulfoximine (BSO) and GSH-monoethyl ester (GSH-MEE) were injected into mice via caudal vein to regulate glutathione (GSH) level in lung. Alterations of lu...Continue Reading
Citations
May 11, 2019·Cellular and Molecular Life Sciences : CMLS·Sandra Chánez-ParedesMichael Schnoor
Oct 6, 2020·Oxidative Medicine and Cellular Longevity·Zhuanzhuan QiangHong Jiang
Jun 27, 2020·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Haiying FuMayu Huang
Nov 12, 2019·Biosensors & Bioelectronics·Xiaoxue XieHaowen Huang
Feb 10, 2021·Free Radical Research·Xia LiJun Hu
Dec 17, 2020·Frontiers in Physiology·Shuai ZhaoYuan Zhang
Dec 29, 2020·Frontiers in Pharmacology·Jing LiJianwu Long
Mar 5, 2021·Medical Oncology·Ying ZhouJianxin Lyu
May 25, 2021·Frontiers in Medicine·Mengdi QuChanghong Miao
Jun 24, 2021·Immunopharmacology and Immunotoxicology·Weixin LiRuiyun Li
Jul 5, 2021·Cell Death & Disease·Qinjie LiuJianan Ren
Jul 27, 2021·BioMed Research International·Qian DouHua Gan
Jul 15, 2021·Cardiovascular Drugs and Therapy·Xiyu ZhangYan Meng
Aug 24, 2021·Oxidative Medicine and Cellular Longevity·Yi-Fei ChenMing Xu
Aug 24, 2021·Frontiers in Pediatrics·Baiye XuZhen Chen
May 19, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qiuhua ChenFengjie Huang