Picosecond and steady state, variable intensity and variable temperature emission spectroscopy of bacteriorhodopsin

Biophysical Journal
S L ShapiroW Stoeckenius

Abstract

The bacteriorhodopsin emission lifetime at 77 degrees K has been obtained for different regions of the emission spectrum with single-pulse excitation. The data under all conditions yield a lifetime of 60 +/- 15 ps. Intensity effects on this lifetime have been ruled out by studying the relative emission amplitude as a function of the excitation pulse energy. We relate our lifetime to previously reported values at other temperatures by studying the relative emission quantum efficiency as a function of temperature. These variable temperature studies have indicated that an excited state with an emission maximum at 670 nm begins to contribute to the spectrum as the temperature is lowered. Within our experimental error the picosecond data seem to suggest that this new emission may arise from a minimum of the same electronic state responsible for the 77 degrees K emission at 720 nm. A correlation is noted between a 1.0-ps formation time observed in absorption by Ippen et al. (Ippen, E.P., C.V. Shank, A. Lewis, and M.A. Marcus. 1978. Subpicosecond spectroscopy of bacteriorhodopsin. Science [wash. D.C.]. 200:1279-1281 and a time extrapolated from relative quantum efficiency measurements and the 77 degrees K fluorescence lifetime that we...Continue Reading

Citations

Jul 21, 2006·The Journal of Physical Chemistry. B·David W McCamantRichard A Mathies
Nov 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Piero AltoèMarco Garavelli
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·R González-LuqueM Olivucci
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·K C HassonP A Anfinrud
Apr 27, 1999·Biochimica Et Biophysica Acta·M H Vos, J L Martin
Nov 10, 2011·Journal of the American Chemical Society·Amir WandSanford Ruhman

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