Jan 30, 2004

Picrotoxin increased acetylcholine release from rat cultured embryonic septal neurons

Neuroscience Letters
Takeshi SuzukiK Kawashima


GABA is a major inhibitory neurotransmitter in the mature mammalian brain. In the early stages of brain development, it has been reported that GABA(A) receptor stimulation and the associated increase in Cl(-) conductance lead to membrane depolarization. In this study, we tested the effects of picrotoxin, a GABA(A) receptor Cl(-) channel blocker, on spontaneously released acetylcholine (ACh) from cultured rat embryonic septal cells. Picrotoxin increased spontaneously released ACh. These results indicate that blockade of GABA-activated Cl(-) channel increases neuronal excitability even in an early stage of the development.

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Mentioned in this Paper

Coculture Techniques
GABA-A Receptor
Rats, Wistar
Acetylcholine Sulfate (1: 1)

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