PIEZO1 Channel Is a Potential Regulator of Synovial Sarcoma Cell-Viability

International Journal of Molecular Sciences
Takahisa SuzukiKatsuhiko Muraki

Abstract

Detection of mechanical stress is essential for diverse biological functions including touch, audition, and maintenance of vascular myogenic tone. PIEZO1, a mechano-sensing cation channel, is widely expressed in neuronal and non-neuronal cells and is expected to be involved in important biological functions. Here, we examined the possibility that PIEZO1 is involved in the regulation of synovial sarcoma cell-viability. Application of a PIEZO1 agonist Yoda1 effectively induced Ca2+ response and cation channel currents in PIEZO1-expressing HEK (HEK-Piezo1) cells and synovial sarcoma SW982 (SW982) cells. Mechanical stress, as well as Yoda1, induced the activity of an identical channel of conductance with 21.6 pS in HEK-Piezo1 cells. In contrast, Yoda1 up to 10 μM had no effects on membrane currents in HEK cells without transfecting PIEZO1. A knockdown of PIEZO1 with siRNA in SW982 cells abolished Yoda1-induced Ca2+ response and significantly reduced cell cell-viability. Because PIEZO1 is highly expressed in SW982 cells and its knockdown affects cell-viability, this gene is a potential target against synovial sarcoma.

References

Jan 18, 2005·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E L SpurrellI R Judson
Apr 20, 2005·Journal of Pediatric Hematology/oncology·Cynthia E Herzog
Dec 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Janet M TseLance L Munn
Feb 22, 2012·Nature·Bertrand CosteArdem Patapoutian
Mar 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chilman BaePhilip A Gottlieb
Apr 11, 2014·Nature·Seung-Hyun WooArdem Patapoutian
Apr 25, 2014·The Journal of Biological Chemistry·Tatsuya MiyamotoMakoto Tominaga
Jun 20, 2014·American Journal of Physiology. Cell Physiology·Hiroka SuzukiKatsuhiko Muraki
Jun 25, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sanjeev S RanadeArdem Patapoutian
Dec 5, 2014·Nature·Sanjeev S RanadeArdem Patapoutian
Feb 11, 2015·Scientific Reports·Chouyang LiWolfgang Schreibmayer
May 23, 2015·ELife·Stuart M CahalanArdem Patapoutian
May 23, 2015·ELife·Ruhma SyedaArdem Patapoutian
Oct 17, 2016·Trends in Biochemical Sciences·Jason WuJörg Grandl
Nov 1, 2016·The Journal of Clinical Investigation·ShengPeng WangStefan Offermanns
Aug 15, 2017·Pharmacology Research & Perspectives·Katsuhiko MurakiYukiko Muraki

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Citations

Oct 11, 2019·International Journal of Molecular Sciences·Maki YonedaKatsuhiko Muraki
Aug 5, 2019·Pflügers Archiv : European journal of physiology·Thiha AungSilke Haerteis
Mar 11, 2020·Journal of Cancer Research and Clinical Oncology·Yanhua SunGuiying Wang
Oct 2, 2020·Scientific Reports·Wenjianlong ZhouWang Jia
Feb 13, 2021·BioMed Research International·Daxue ZhuXuewen Kang
Apr 6, 2019·Biochemical and Biophysical Research Communications·Ying-Ying WangYong-Wang Li
Mar 27, 2021·Molecular Therapy. Nucleic Acids·Biqiang ZhuXiaohua Hou
May 4, 2021·Frontiers in Pharmacology·Jing GuoJianhua Sun
May 19, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jia-Lin Yu, Hai-Yang Liao

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Methods Mentioned

BETA
FCS
transfection
PCR
Fluorescence

Software Mentioned

WINEDR3
WinWCPV4
Imagework Bench

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