Piezoelectric printing and probing of Lectin NanoProbeArrays for glycosylation analysis

Biochemical and Biophysical Research Communications
Vinay Janthakahalli NagarajPeter Wiktor

Abstract

Glycans have great potential as disease biomarkers and therapeutic targets. However, the major challenge for glycan biomarker identification from clinical samples is the low abundance of key glycosylated proteins. To demonstrate the potential for glycan analysis with nanoliter amounts of glycoprotein, we have developed a new technology (Lectin NanoProbeArray) based on piezoelectric liquid dispensing for non-contact printing and probing of a lectin array. Instead of flooding the glycoprotein probe on the lectin array surface, as in conventional microarray screening, a piezoelectric printer is used to dispense nanoliters of fluorescently labeled glycoprotein probe over the lectin spots on the array. As a proof-of-concept, the ability of Lectin NanoProbeArrays to precisely identify and reliably distinguish between the closely related glycoforms of fetuin is illustrated here. Sensitivity levels comparable to lectin arrays that use evanescent-field scanners was achieved along with several orders of magnitude reduction in the amount of probe required for glycosylation analysis.

References

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Citations

Aug 24, 2010·Omics : a Journal of Integrative Biology·Garima GuptaSrinivasa-Gopalan Sampathkumar
Feb 5, 2010·Biological Chemistry·Dieter VanderschaegheNico Callewaert
Apr 17, 2010·Nanomedicine·Vinay J NagarajShalini Prasad
May 10, 2012·Journal of Structural Biology·Tilak JainClinton S Potter
Sep 1, 2009·Current Opinion in Chemical Biology·Ku-Lung Hsu, Lara K Mahal
Jan 16, 2010·Mass Spectrometry Reviews·Tasneem PatwaDavid M Lubman
Mar 5, 2015·Scientific Reports·Peter WiktorJoshua LaBaer
May 26, 2011·Journal of Laboratory Automation·Vinay Janthakahalli NagarajPeter Wiktor

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