PIF direct immune regulation: Blocks mitogen-activated PBMCs proliferation, promotes TH2/TH1 bias, independent of Ca(2+)

Immunobiology
Eytan R BarneaNelson Fernández

Abstract

PreImplantation Factor (PIF(9&15)) secreted by viable embryos exerts an essential transplant acceptance and immune regulatory role in pregnancy. Synthetic PIF replicates endogenous PIF's effect in pregnant and non-pregnant immune disorder models. PIF binds macrophages to regulate CD3/CD28-induced T-cell response. We present evidence that PIF regulates the co-stimulatory T-cell receptor, CD2, which binds to and is activated by phytohemagglutinin (PHA), a potent mitogen, confirming PIF's ability to systemically respond to diverse immune stimulants. PIF's effect on PHA-activated PBMC (male and non-pregnant females) proliferation and cytokine secretion was tested, showing that both PIF(9&15) block PHA-induced PBMC proliferation and promote anti-inflammatory IL10 secretion, while reducing pro-inflammatory IFNγ secretion. Thus favoring a T(H)2 cytokine bias. Surface plasmon resonance spectroscopy, immunocytochemistry and Flex station experiments reveal that PIF effect is direct. PIF targets intracellular targets but does not affect early Ca(2+) mobilization. By promoting the CD2 receptor in activated T-cells and through inhibition of co-ligand CD58 expression, PIF regulates antigen-presenting cell (APC)-T-cell interactions required f...Continue Reading

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Citations

Aug 6, 2015·Journal of Obstetrics and Gynaecology of India·Gautam N Allahbadia
Dec 3, 2016·Cell Death & Disease·Hadia MoindjieMarie-Noëlle Dieudonné
May 12, 2020·The International Journal of Biological Markers·Maria Rosaria RaspolliniEytan R Barnea
Aug 15, 2020·Alimentary Pharmacology & Therapeutics·Neil HallidayMichael A Heneghan
Apr 2, 2020·Frontiers in Immunology·Salwan Al-NasiryBoris W Kramer

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