Pig kidney dopa decarboxylase: inactivation by iodoacetamide and sequence of the carboxyamidomethylcysteine-containing peptide

Journal of Enzyme Inhibition
P DominiciC Borri Voltattorni

Abstract

Pig kidney 3,4-dihydroxyphenylalanine (Dopa) decarboxylase is inactivated by iodoacetamide following pseudo-first order reaction kinetics. The apparent first order rate constant for inactivation is proportional to the concentration of iodoacetamide and a second order rate constant of 37 M-1 min-1 is obtained at pH 6.8 and 25 degrees C. Cyanogen bromide fragmentation of iodo(1-14C)acetamide - modified inactivated Dopa decarboxylase followed by trypsin digestion yields a single radioactive peptide. Automated Edman degradation reveals a heptapeptide sequence which contains labeled carboxyamidomethylcysteine. This finding and the results of the incorporation of the label from ido (1-14C)acetamide into the enzyme clearly indicate that the modification of 1 mol of SH per mol of enzyme dimer is responsible for the inactivation process. The labeled peptide, which was located by means of limited proteolysis on the fragment corresponding to the COOH-terminal third of the enzyme, has been aligned with a 7 amino acid stretch of Drosophila enzyme. Although this region appears highly conserved in the Dopa decarboxylase enzymes, the cysteinyl residue is not conserved. This observation together with the spectral binding properties of the iodoa...Continue Reading

References

Jun 1, 1975·Analytical Biochemistry·A Charteris, R John
Jan 2, 1979·European Journal of Biochemistry·C B VoltattorniC Turano
Sep 9, 1977·Biochemical and Biophysical Research Communications·F BossaC Turano
Jan 1, 1987·Methods in Enzymology·T L Sourkes
Jan 1, 1987·Methods in Enzymology·C B VoltattorniC Turano
May 1, 1985·Archives of Biochemistry and Biophysics·B TanciniC B Voltattorni
Nov 1, 1973·Analytical Biochemistry·A F SheraldT R Wright
Nov 1, 1970·Archives of Biochemistry and Biophysics·J G ChristensonS Udenfriend
May 16, 1967·Biochimica Et Biophysica Acta·C Streffer

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