Jul 1, 1975

Pigeon-liver NAD kinase. The structural and kinetic basis of regulation of NADPH

European Journal of Biochemistry
David K. Apps


NAD kinase was purified from pigeon liver by an improved procedure which included chromatography on phosphocellulose. The resultant preparation was homogeneous as judged by gel electrophoresis, but electrofocusing gave indications of heterogeneity. The enzyme appeared to be of molecular weight 270000, and to consist of subunits of molecular weight 34000; it may therefore be an octomer. Kinetic studies over a wide range of substrate concentrations revealed departures from Michaelis-Menten behaviour with the substrate NAD+; these were interpreted tentatively in terms of negative homotropic interactions between identical binding sites, since thermal and chemical inactivation studies revealed no evidence for more than one type of catalytic site. The significance of the kinetics and of the type of inhibition produced by NADPH is discussed in terms of the regulation of NAD kinase activity in vivo.

Mentioned in this Paper

In Vivo
Macromolecular Compounds
NAD+ kinase
Regulation of Biological Process
Allergy Testing Pigeon
Regulation of NAD+ Kinase Activity

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