Pigeonpea Hybrid-Proline-Rich Protein (CcHyPRP) Confers Biotic and Abiotic Stress Tolerance in Transgenic Rice

Frontiers in Plant Science
Sunitha MellacheruvuVenkateswara Rao Khareedu

Abstract

In this study, we report the overexpression of Cajanus cajan hybrid-proline-rich protein encoding gene (CcHyPRP) in rice which resulted in increased tolerance to both abiotic and biotic stresses. Compared to the control plants, the transgenic rice lines, expressing CcHyPRP, exhibited high-level tolerance against major abiotic stresses, viz., drought, salinity, and heat, as evidenced by increased biomass, chlorophyll content, survival rate, root, and shoot growth. Further, transgenic rice lines showed increased panicle size and grain number compared to the control plants under different stress conditions. The CcHyPRP transgenics, as compared to the control, revealed enhanced activities of catalase and superoxide dismutase (SOD) enzymes and reduced malondialdehyde (MDA) levels. Expression pattern of CcHyPRP::GFP fusion-protein confirmed its predominant localization in cell walls. Moreover, the CcHyPRP transgenics, as compared to the control, exhibited increased resistance to the fungal pathogen Magnaporthe grisea which causes blast disease in rice. Higher levels of bZIP and endochitinase transcripts as well as endochitinase activity were observed in transgenic rice compared to the control plants. The overall results demonstrate t...Continue Reading

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Citations

Nov 22, 2019·Frontiers in Plant Science·Zihan ChengTingbo Jiang
May 30, 2019·International Journal of Molecular Sciences·Jinhua LiXingguo Zhang
May 8, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Banashree SaikiaChannakeshavaiah Chikkaputtaiah
Mar 17, 2020·Phytopathology·Chitathoor Balasubramane Sruthilaxmi, Subramanian Babu
Jan 12, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Banashree SaikiaChannakeshavaiah Chikkaputtaiah
Nov 26, 2019·Plant Physiology and Biochemistry : PPB·Michele Valquíria Dos ReisSchuyler S Korban
May 1, 2021·Frontiers in Microbiology·Bishnu Maya BashyalRashmi Aggarwal
Jun 3, 2021·International Journal of Molecular Sciences·Xiaoli ZhangFengwang Ma
Jul 13, 2021·3 Biotech·Nunna Sai Aparna DeviRamasamy Krishnamoorthy

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Methods Mentioned

BETA
environmental stresses
transgenic
MDA
protein assay
PCR
transgenics

Software Mentioned

Excel

Related Concepts