piggyBac-mediated somatic transformation of the two-spotted cricket, Gryllus bimaculatus

Development, Growth & Differentiation
Yohei ShinmyoSumihare Noji

Abstract

Transgenic insects have been artificially produced to study functions of interesting developmental genes, using insect transposons such as piggyBac. In the case of the cricket, however, transgenic animals have not yet been successfully artificially produced. In the present study, we examined whether the piggyBac transposon functions as a tool for gene delivery in embryos of Gryllus bimaculatus. We used either a piggyBac helper plasmid or a helper RNA synthesized in vitro as a transposase source. An excision assay revealed that the helper RNA was more effective in early Gryllus eggs to transpose a marker gene of eGFP than the helper plasmid containing the piggyBac transposase gene driven by the G. bimaculatus actin3/4 promoter. Further, only when the helper RNA was used, somatic transformation of the embryo with the eGFP gene was observed. These results suggest that the piggyBac system with the helper RNA may be effective for making transgenic crickets.

References

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Apr 19, 2002·Mechanisms of Development·Katsuyuki MiyawakiSumihare Noji
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Alfred M Handler
Oct 24, 2002·Development, Growth & Differentiation·Hongjie ZhangSumihare Noji

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Citations

Sep 28, 2013·BMC Biotechnology·Solenne BireFlorence Rouleux-Bonnin
Apr 11, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Philip F Copenhaver
Jun 15, 2012·Archives of Insect Biochemistry and Physiology·Dan LiuQisheng Song
Dec 19, 2009·Insect Molecular Biology·J WangY Park
Mar 18, 2008·Insect Biochemistry and Molecular Biology·Jianjun WangYoonseong Park
Oct 29, 2013·Journal of Insect Physiology·Chihiro Sato MatsumotoHiroto Ogawa
Jun 9, 2016·Current Opinion in Genetics & Development·Marco GrilloMichalis Averof
Nov 21, 2017·Genes & Genetic Systems·Ayumi KudoTakashi Matsuo
Dec 13, 2005·Nature Reviews. Genetics·Andrew D PeelMichael Akam
Apr 26, 2011·Molecular and Cellular Biochemistry·Adele Kim, Ilmari Pyykko
Jun 26, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria E MoralesPaul J Brindley
Jun 25, 2015·Microbiology Spectrum·Kosuke Yusa
Jun 16, 2021·Communications Biology·Guillem YllaCassandra G Extavour

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