piggyBac transposon-mediated long-term gene expression in mice.

Molecular Therapy : the Journal of the American Society of Gene Therapy
Hideyuki NakanishiMitsuru Hashida

Abstract

Transposons are promising systems for somatic gene integration because they can not only integrate exogenous genes efficiently, but also be delivered to a variety of organs using a range of transfection methods. piggyBac (PB) transposon has a high transposability in mammalian cells in vitro, and has been used for genetic and preclinical studies. However, the transposability of PB in mammalian somatic cells in vivo has not been demonstrated yet. Here, we demonstrated PB-mediated sustained gene expression in adult mice. We constructed PB-based plasmid DNA (pDNA) containing reporter [firefly and Gaussia luciferase (Gluc)] genes. Mice were transfected by injection of these pDNAs using a hydrodynamics-based procedure, and the conditions for high-level sustained gene expression were examined. Consequently, gene expressions were sustained over 2 months. Our results suggest that PB is useful for organ-selective somatic integration and sustained gene expression in mammals, and will contribute to basic genetic studies and gene therapies.

References

Jul 5, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Aron M GeurtsPerry B Hackett
Aug 30, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Lalitha R BelurR Scott McIvor
Feb 5, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Zsuzsanna Izsvák, Zoltán Ivics
Apr 15, 2004·Biochemical and Biophysical Research Communications·Keita MasudaYasufumi Kaneda
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bakhos A TannousXandra O Breakefield
Nov 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Corey M CarlsonDavid A Largaespada
Jan 18, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·William J BowersHoward J Federoff
May 3, 2006·Human Molecular Genetics·Adam J DupuyNeal G Copeland
Jul 11, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Naohiko FujiiEnyu Imai
Aug 1, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K J MaragathavallyC J Coates
Sep 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sareina Chiung-Yuan WuJoseph M Kaminski
Oct 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hanzhong LiuGary A Visner
Nov 1, 2006·Current Gene Therapy·Zoltán Ivics, Zsuzsanna Izsvák
Dec 14, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Matthew H WilsonAlfred L George
Mar 9, 2007·Nucleic Acids Research·Stephen R YantMark A Kay
Apr 12, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Zoltán IvicsZsuzsanna Izsvák
Jun 20, 2007·Nucleic Acids Research·Juan Cadiñanos, Allan Bradley
Jul 21, 2007·BMC Biotechnology·Karl J ClarkScott C Fahrenkrug
Sep 8, 2007·Journal of Pharmaceutical Sciences·Shigeru KawakamiMitsuru Hashida
Oct 16, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shyh-Dar Li, Leaf Huang
Oct 17, 2007·Molecular and Cellular Biology·Brian S GarrisonMark A Kay
Dec 6, 2007·Genome Biology·Junji TakedaKyoji Horie
Jan 22, 2008·Nature Methods·Thomas WurdingerBakhos A Tannous
May 1, 2008·Biochemical and Biophysical Research Communications·Hidefumi MukaiMitsuru Hashida
Jun 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Wei WangPentao Liu
Jan 17, 2009·Cancer Research·Stephen M WiesnerJohn R Ohlfest
Feb 26, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Nicklas H StaunstrupJacob Giehm Mikkelsen
May 7, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Conrad A VinkWaseem Qasim
May 19, 2009·Human Gene Therapy·Hidefumi MukaiMitsuru Hashida

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Citations

Dec 14, 2011·Analytical Chemistry·M Sarah S BovenbergBakhos A Tannous
Jan 25, 2013·Molecular Therapy. Nucleic Acids·Erin R BurnightPaul B McCray
Oct 14, 2011·Human Gene Therapy·Joseph E DohertyMatthew H Wilson
May 10, 2011·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Yu TaniguchiAtsushi Kamiya
Apr 3, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yuriko Higuchi
May 19, 2011·Yi chuan = Hereditas·Lin LiuYue Huang
Nov 15, 2012·Journal of Visualized Experiments : JoVE·Sunandan SahaMatthew H Wilson
Oct 26, 2013·Nanomedicine·Seog-Jin SeoHae-Won Kim
Oct 18, 2013·Gene Therapy·V SolodushkoB Fouty
Jul 19, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mario Di MatteoThierry VandenDriessche
Jun 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xianghong LiNancy L Craig
Sep 28, 2013·BMC Biotechnology·Solenne BireFlorence Rouleux-Bonnin
Sep 24, 2015·Journal of Drug Targeting·Yota TaniguchiMitsuru Hashida
Jun 12, 2012·Expert Opinion on Biological Therapy·Mario Di MatteoThierry Vandendriessche
Sep 17, 2013·Ultrasound in Medicine & Biology·Cynthia D AndersonChad B Walton
Jun 7, 2011·Journal of Biotechnology·Hideyuki NakanishiMitsuru Hashida
Sep 14, 2014·Seminars in Nephrology·Minoru TakasatoMelissa H Little
Jul 28, 2015·Trends in Biotechnology·Lauren E Woodard, Matthew H Wilson
Apr 1, 2015·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Rajagopal N AravalliClifford J Steer
Jun 24, 2015·Human Gene Therapy·Kristian Alsbjerg Skipper, Jacob Giehm Mikkelsen
Sep 17, 2011·Biotechnology Advances·Bakhos A Tannous, Jian Teng
Jul 14, 2012·Biological & Pharmaceutical Bulletin·Yoshiaki UmemotoMitsuru Hashida
Apr 9, 2015·Frontiers in Neuroscience·Maria Figueres-OñateLaura López-Mascaraque
Jun 24, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Tomoaki KurosakiMitsuru Hashida
Jan 6, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ashley L CooneyPatrick L Sinn
Sep 1, 2012·Biochemical Pharmacology·Shaina R EckhouseFrancis G Spinale
Oct 13, 2016·Journal of Translational Medicine·José Eduardo VargasMartin H Bonamino
Sep 3, 2016·International Journal of Molecular Sciences·Masahiro SatoKazuchika Miyoshi
Jun 17, 2020·JCI Insight·Mauro CastellarinCarl H June
Aug 14, 2020·Genes·Carlos G Moscoso, Clifford J Steer
Jun 30, 2019·International Journal of Molecular Sciences·Masahiro SatoSatoshi Watanabe
Jul 29, 2020·Human Gene Therapy·Christian M BrommelPatrick L Sinn

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Methods Mentioned

BETA
xenograft
transfection
PCR

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