PMID: 9654062Jul 8, 1998Paper

Pigment-binding properties of the recombinant photosystem II subunit CP26 reconstituted in vitro

European Journal of Biochemistry
F RosH Paulsen

Abstract

CP26 is the most recently described antenna protein in higher plants which has been reported to be involved in xanthophyll-dependent regulation of the light-harvesting function but is largely unknown due to the difficulties of purification. In this study we have overexpressed in Escherichia coli the Lhcb5 gene product and reconstituted CP26 in vitro by refolding the recombinant protein in the presence of chlorophyll a, chlorophyll b and xanthophylls. The resulting pigment-protein complex is stable enough to be isolated by partially denaturing gel electrophoresis. Reconstitution and isolation conditions for CP26 are similar to those used for other chlorophyll a/b complexes like the major light-harvesting complex of photosystem II (LHCII) and CP29; however, CP26 differs with regard to its lower specificity in carotenoid binding. Most significantly, rather stable recombinant CP26 can be reconstituted containing violaxanthin as the only carotenoid. This enhanced plasticity with respect to carotenoid binding is consistent with CP26 being the major binding protein of violaxanthin involved in the xanthophyll cycle. The availability of recombinant CP26 opens the way to a better characterisation of this pigment-protein complex with rega...Continue Reading

Citations

Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·B J Pogson, H M Rissler
Mar 31, 2009·Biochimica Et Biophysica Acta·Tiago Barros, Werner Kühlbrandt
Feb 27, 1999·Current Opinion in Structural Biology·P J Booth, A R Curran
Jun 28, 2011·Biochimica Et Biophysica Acta·Matteo BallottariRoberto Bassi
Apr 6, 2002·The Journal of Biological Chemistry·Paola DominiciRoberto Bassi
Jul 4, 2002·The Journal of Biological Chemistry·Volkmar H R SchmidStefanie Storf
Oct 9, 1999·The Journal of Biological Chemistry·R CroceR Bassi
Nov 26, 2004·The Journal of Biological Chemistry·Stefanie StorfVolkmar H R Schmid
Jun 7, 2006·The Journal of Biological Chemistry·Antje WehnerPeter Jahns
Oct 10, 2018·Nature Plants·Daniel M PalmHarald Paulsen
Dec 19, 2002·The Journal of Biological Chemistry·Stephan HobeHarald Paulsen

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