Pigment configuration in the light-harvesting protein of the xanthophyte alga Xanthonema debile

Photosynthesis Research
Simona StreckaiteManuel J Llansola-Portoles

Abstract

The soil chromophyte alga Xanthonema (X.) debile contains only non-carbonyl carotenoids and Chl-a. X. debile has an antenna system denoted Xanthophyte light-harvesting complex (XLH) that contains the carotenoids diadinoxanthin, heteroxanthin, and vaucheriaxanthin. The XLH pigment stoichiometry was calculated by chromatographic techniques and the pigment-binding structure studied by resonance Raman spectroscopy. The pigment ratio obtained by HPLC was found to be close to 8:1:2:1 Chl-a:heteroxanthin:diadinoxanthin:vaucheriaxanthin. The resonance Raman spectra suggest the presence of 8-10 Chl-a, all of which are 5-coordinated to the central Mg, with 1-3 Chl-a possessing a macrocycle distorted from the relaxed conformation. The three populations of carotenoids are in the all-trans configuration. Vaucheriaxanthin absorbs around 500-530 nm, diadinoxanthin at 494 nm and heteroxanthin at 487 nm at 4.5 K. The effective conjugation length of heteroxanthin and diadinoxanthin has been determined as 9.4 in both cases; the environment polarizability of the heteroxanthin and diadinoxanthin binding pockets is 0.270 and 0.305, respectively.

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Citations

Feb 6, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Simona StreckaiteBruno Robert
Mar 13, 2020·The Journal of Physical Chemistry. a·Simona StreckaiteManuel J Llansola-Portoles
Jun 4, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Claudia Büchel
Sep 3, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Manuel J Llansola-PortolesBruno Robert
May 15, 2021·Journal of Experimental Botany·Vesa HavurinneEsa Tyystjärvi
Feb 4, 2022·The Journal of Physical Chemistry. a·Mindaugas MacernisLeonas Valkunas

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Methods Mentioned

BETA
density gradient centrifugation

Software Mentioned

MP

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