PMID: 58996Jun 9, 1976

Pigment containing lipid vesicles. I. Preparation and characterization of chlorophyll a-lecithin vesicles

The Journal of Membrane Biology
E Ritt, D Walz


Vesicles obtained by sonication of chlorophyll a-lecithin mixtures dispersed in anaqueous medium closely resemble the well-characterized vesicles similarly prepared from pure lipids. They are bounded by one spherical lipid bilayer which contains the chlorophyll a. Appropriate conditions for sonication prevent substantial degradation of the membrane constituents. Up to one chlorophyll a molecule per 55 lecithins can be incorporated into membranes. The average Stokes' radius of the vesicles determined by analytical sieve chromatography is 102 +/- 5 A and independent of the chloropyll a content. The membrane is visible in the electron-microscope when the vesicles are treated with osmium tetroxide prior to negative staining. The osmium fixation is, however, not strong enough to allow for a preparation of the vesicles for thin sectioning (dehydration, embedding in epoxide).


Apr 21, 1975·Biochimica Et Biophysica Acta·J N Israelachvili, D J Mitchell
Jun 1, 1971·Biochimica Et Biophysica Acta·S M JohnsonE D Korn
Mar 30, 1971·Biochemistry·R D Kornberg, H M McConnell
Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R D Kornberg, H M McConnell
Jan 1, 1972·Journal of Ultrastructure Research·R B Litman, R J Barrnett
Nov 21, 1972·Biochemistry·M P Sheetz, S I Chan
Oct 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H O Hauser, L Irons
Aug 9, 1973·Biochimica Et Biophysica Acta·K Colbow
Nov 1, 1971·Biochemical and Biophysical Research Communications·H O Hauser
Sep 19, 1970·Nature·H T Tien, S P Verma
Dec 8, 1970·Biochimica Et Biophysica Acta·T TrosperB Ke
Sep 8, 1970·Biochimica Et Biophysica Acta·R A Klein
Apr 16, 1971·Biochemical and Biophysical Research Communications·R J CherryD Chapman
Apr 12, 1968·Science·D Chapman, P G Fast
Sep 27, 1965·Biochimica Et Biophysica Acta·H H StrainJ J Katz
Jan 1, 1965·Journal of the American Oil Chemists' Society·W S SINGLETONJ L WHITE
Dec 31, 1949·Nature·C S HANES, F A ISHERWOOD
Dec 1, 1970·The Journal of Membrane Biology·J P Reeves, R M Dowben
Dec 1, 1971·The Journal of Membrane Biology·V K Miyamoto, W Stoeckenius
Dec 1, 1971·The Journal of Membrane Biology·A SteinemannP Läuger
Dec 1, 1972·The Journal of Membrane Biology·A SteinemannP Läuger


Jan 30, 1981·The Journal of Membrane Biology·F Bellemare, M Fragata

Related Concepts

Membranes, Artificial
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