Pigment epithelium-derived factor and interleukin-6 control prostate neuroendocrine differentiation via feed-forward mechanism.

The Journal of Urology
Norm D SmithOlga V Volpert

Abstract

PEDF (pigment epithelium-derived factor) promotes the differentiation and survival of neuronal cells, and expands the adult neuronal stem cell niche. In the prostate PEDF is suppressed by androgen with unclear physiological consequences. We report that PEDF induced the neuroendocrine differentiation of prostate cancer cells, which was accompanied by neurite outgrowth and chromogranin A expression. We performed neuroendocrine differentiation assay, Western blot analysis, immunostaining and reverse transcriptase-polymerase chain reaction in the human prostate cancer cell lines LNCaP, PC-3 and DU145, and the prostate epithelial strain RWPE-1 (ATCC). Ectopic and endogenous PEDF caused neuroendocrine differentiation of prostate cancer cells, as manifested by neurite-like outgrowths and chromogranin A expression. The transdifferentiated cells expressed axonal and dendritic markers, as ascertained by immunoblotting for specific markers. Neuroendocrine cells formed multiple synaptophysin positive protrusions resembling dendritic spines and vesicles containing serotonin, pointing to possible synapse formation. The known transdifferentiating agent interleukin-6 induced PEDF secretion. Moreover, PEDF neutralizing antibodies abolished the ...Continue Reading

References

Jan 5, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P A di Sant'Agnese
Jan 5, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J Hansson, P A Abrahamsson
Jul 9, 2004·International Journal of Cancer. Journal International Du Cancer·Qingcai WangJacek Pinski
Aug 4, 2004·Cancer Research·Ren Jie JinRobert J Matusik
Jun 23, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Joyce Tombran-Tink
Sep 28, 2005·Neurochemical Research·Mark P Mattson
Dec 20, 2005·Current Opinion in Neurobiology·Tomoko Tada, Morgan Sheng
Feb 24, 2006·Nature Neuroscience·Carmen Ramírez-CastillejoIsabel Fariñas
Apr 25, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Anne T Collins, Norman J Maitland
May 3, 2006·The Prostate·Jacek PinskiPeter V Danenberg
Jul 18, 2006·Urology·Amir H Shariff, M Hammad Ather

❮ Previous
Next ❯

Citations

Jul 6, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Joseph L SottnikEvan T Keller
Mar 15, 2013·Nature Reviews. Cancer·S Patricia Becerra, Vicente Notario
Feb 19, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yelena MirochnikOlga V Volpert
May 7, 2013·Expert Opinion on Drug Discovery·Susan E CrawordOlga V Volpert
Jan 10, 2016·Journal of Experimental & Clinical Cancer Research : CR·Louiza Belkacemi, Shaun Xiaoliu Zhang
Jan 31, 2009·Journal of Cellular Biochemistry·S FilleurR C Kennedy
Sep 28, 2014·Biochemical Pharmacology·Qing GongZhonghan Yang
Feb 24, 2015·Frontiers in Oncology·Panagiotis J Vlachostergios, Christos N Papandreou
Mar 10, 2015·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Guilherme Silva JulianJair Ribeiro Chagas
Mar 21, 2019·Current Pharmaceutical Design·Sho-Ichi YamagishiMinoru Yagi
Mar 16, 2017·Chinese Journal of Cancer·Jinlu DaiEvan T Keller
Oct 12, 2018·Endocrine-related Cancer·S PrekovicF Claessens

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.