Pigment gallstone dissolution in vitro. Solubilization of brown bilirubinate and black polybilirubinate stone material by buffered solvents containing ethylenediaminetetraacetic acid, bile salts, and reducing thiols

Scandinavian Journal of Gastroenterology
U WosiewitzU Leuschner

Abstract

The efficacy of a buffered 1% ethylenediaminetetraacetic acid (EDTA)-2Na solution (pH 9.2) in solubilizing carefully pulverized material from brown bilirubinate and black polybilirubinate pigment stones can be intensified stepwise by admixtures of detergents and mucolytic active thiols. Solubilization effects were quantified either photometrically by measuring the dissolved calcium bilirubinate or gravimetrically by measuring the total weight loss of solids after a defined incubation period. Maximum effects were achieved when using a buffered solvent with 1 g/dl disodium-EDTA, 1 g/dl sodium taurocholate (NaTCA), and 2 g/dl N-acetylcysteine (NAC). Whereas admixtures of NAC enhanced the solubilization of brown bilirubinate stone material additionally by an average of 21.3% (related to the effect of an EDTA/NaTCA-containing solvent), black polybilirubinate material responded rather poorly and inconsistently to NAC (mean, 8.4 +/- 11.7%).

References

Jun 1, 1978·The Tohoku Journal of Experimental Medicine·Y NakamuraT Sato
Jun 15, 1979·Experientia·U Wosiewitz, S Schroebler
Oct 2, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·U Wosiewitz, S Schroebler
Feb 1, 1987·American Journal of Surgery·H A PittT R Gadacz
Jan 1, 1985·Gastroenterology·M J AllenJ L Thistle
Jan 1, 1983·Digestion·U WosiewitzB Schmack
May 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J T LaMontR D Soloway
Sep 7, 1982·Biochimica Et Biophysica Acta·J P PearsonA Allen
Mar 1, 1981·The British Journal of Surgery·R W Motson

❮ Previous
Next ❯

Citations

Feb 1, 1991·Digestive Diseases and Sciences·U LeuschnerM Leuschner
Jun 1, 1994·Digestive Diseases and Sciences·U LeuschnerM Leuschner
Nov 1, 1991·Journal of Internal Medicine·U Leuschner
Jan 1, 1994·Journal of Gastroenterology and Hepatology·U LeuschnerA Hellstern
Jun 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M OkidoF Nakayama
Mar 23, 2004·The Veterinary Clinics of North America. Small Animal Practice·Sharon A Center

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.