Pigmentation Is Associated with Stemness Hierarchy of Progenitor Cells Within Cultured Limbal Epithelial Cells

Stem Cells
Lei LiuVladimir Zachar

Abstract

Ex vivo cultured human limbal epithelial stem/progenitor cells (hLESCs) are the main source for regenerative therapy of limbal stem cell deficiency (LSCD), which is worldwide one of the major causes of corneal blindness. Despite many stemness-associated markers have been identified within the limbal niche, the phenotype of the earliest hLESCs has not been hitherto identified. We sought to confirm or refute the use of tumor protein p63 (p63) and ATP binding cassette subfamily B member 5 (ABCB5) as surrogate markers for hLESCs early within the limbal differentiation hierarchy. Based on a robust fluorescence-activated cell sorting and subsequent RNA isolation protocol, a comprehensive transcriptomic profile was obtained from four subpopulations of cultured hLESCs. The subpopulations were defined by co-expression of two putative stem/progenitor markers, the p63 and ABCB5, and the corneal differentiation marker cytokeratin 3. A comparative transcriptomic analysis yielded novel data that indicated association between pigmentation and differentiation, with the p63 positive populations being the most pigmented and immature of the progenitors. In contrast, ABCB5, either alone or in co-expression patterns, identified more committed proge...Continue Reading

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Citations

Aug 23, 2018·Stem Cells·Majlinda Lako
Jul 8, 2020·Scientific Reports·Naresh PolisettiUrsula Schlötzer-Schrehardt
Feb 13, 2021·Genesis : the Journal of Genetics and Development·Mohd Tayyab Adil, Jonathan J Henry
Mar 7, 2021·Progress in Retinal and Eye Research·Clémence BonnetSophie X Deng
May 19, 2021·Translational Vision Science & Technology·Damien GuindoletGilles Thuret
Aug 24, 2021·The Ocular Surface·Naresh PolisettiUrsula Schlötzer-Schrehardt

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