PMID: 2120778Jul 1, 1990Paper

Pigmented cell lines of mouse albino melanocytes containing a tyrosinase cDNA with an inducible promoter

Somatic Cell and Molecular Genetics
L Larue, B Mintz

Abstract

Melanocyte cell lines, with characteristic dendritic morphology and melanosomes, were established from young mice of wild-type (C57BL/6) and of two albino (C57BL/6-c2J/c2J and BALB/c) inbred strains. The albino cells were cotransfected with two plasmids: pMTtyr1, containing the full-length tyr1 cDNA for tyrosinase encoded by the c locus, under the control of the inducible mouse metallothionein-I (MT-I) promoter; and pSVneo beta, allowing selection of transformants by G418 resistance. The intrinsic albino defect was corrected by the tyr1 cDNA in transfected cells, thereby validating the coding capability of tyr1 for tyrosinase. Black melanin was formed in the genetically black (B/B) C57BL/6-c2J/c2J cells and brown melanin in the genetically brown (b/b) BALB/c cells. Pigment was produced even without adding heavy metal (for induction of the MT-I promoter), thus obviating the need for adding it, but was formed more rapidly upon addition of ZnSO4 up to 100 microM. Stable transfected albino melanocyte lines with active tyrosinase and melanization were obtained. Addition of ZnSO4 at 200 microM was lethal to the cells. However, this toxicity--attributable at least in part to melanin precursors--was prevented if the cells sojourned at ...Continue Reading

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Citations

Sep 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·I J Jackson
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M BradlB Mintz
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B Mintz, M Bradl
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L LarueB Mintz
Nov 17, 2011·Pigment Cell & Melanoma Research·Irina BerlinLionel Larue
Dec 18, 2001·Environmental and Molecular Mutagenesis·J Kaur, H Z Hill
Jan 1, 1992·Pigment Cell Research·L B Giebel, R A Spritz

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