PMID: 9428005Jan 15, 1998Paper

Pigmented human skin equivalent: new method of reconstitution by grafting an epithelial sheet onto a non-contractile dermal equivalent

Pigment Cell Research
K NakazawaO Damour

Abstract

We have established a new protocol for reconstituting a pigmented human skin equivalent (PSE) and have evaluated its functional responses to environmental stimulus, UVB. The PSE is reconstituted by grafting an epithelial sheet consisting of keratinocytes and melanocytes onto a porous non-contractile dermal equivalent populated with mitotically and metabolically active fibroblasts. i) The PSE has a multilayered, well-differentiated epidermis with cuboidal basal cells and highly organised dermis with newly synthesised extracellular matrix components. ii) Ki67-positive proliferating keratinocytes (18.1 +/- 7.4%) were detected on the basal layer of the epidermis. iii) Melanocytes located exclusively within the basal layer were detected by monoclonal antibody against tyrosinase-related protein (TRP-1). iv) After exposure to UVB (100 mJ/cm2 per day) for 7 consecutive days, the intensity of TRP-1 staining was increased in the PSE, showing their functional state, whereas the number of melanocytes was not changed. This non-contractile and functioning new PSE is potentially useful as a model for studying the role of melanocyte-keratinocyte-fibroblast interactions in photoprotection of the skin in more complex cutaneous microenvironment t...Continue Reading

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Citations

Jun 15, 1999·Medical & Biological Engineering & Computing·K NakazawaO Damour
Jun 15, 1999·Medical & Biological Engineering & Computing·M RégnierR Schmidt
Nov 14, 2013·Tissue Engineering. Part C, Methods·Tara L FernandezZee Upton
Jun 10, 1998·The Journal of Investigative Dermatology·K NakazawaH Nakazawa
Apr 2, 2008·International Journal of Cosmetic Science·Z WangT Zhang
Jan 7, 2000·The American Journal of Pathology·F MeierM Herlyn
Oct 10, 2002·Applied Immunohistochemistry & Molecular Morphology : AIMM·Nicola R DeanRodney D Cooter
Nov 28, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Viki B SwopeSteven T Boyce
Nov 22, 2005·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·Mutsumi OkazakiKiyonori Harii
Apr 27, 2018·Experimental Dermatology·Alessandra MarconiCarlo Pincelli
Jul 11, 2006·European Journal of Gastroenterology & Hepatology·Tanja SpottlGerhard Rogler
Jun 11, 2002·Analytical Biochemistry·Tie Chi LeiVincent J Hearing

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