Pilot production of recombinant human clotting factor IX from transgenic sow milk

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Yu-ling SunChon-ho Yen

Abstract

Valuable pharmaceutical proteins produced from the mammary glands of transgenic livestock have potential use in the biomedical industry. In this study, recombinant human clotting factor IX (rhFIX) produced from transgenic sow milk for preclinical animal studies have been established. The transgenic sow milk was skimmed and treated with sodium phosphate buffer to remove abundant casein protein. Then, the γ-carboxylated rhFIX fraction was segregated through the Q Sepharose chromatography from uncarboxylated one. For safety issue, the process included virus inactivation by solvent/detergent (S/D) treatment. Subsequently, the S/D treated sample was loaded into the Heparin Sepharose column to recover the rhFIX fraction, which was then reapplied to the Heparin Sepharose column to enhance rhFIX purity and lower the ratio of activated form rhFIX (rhFIXa) easily. This was possible due to the higher affinity of the Heparin affinity sorbent for rhFIXa than for the rhFIX zymogen. Furthermore, an IgA removal column was used to eliminate porcine IgA in purified rhFIX. Finally, nanofiltration was performed for viral clearance. Consequently, a high-quality rhFIX product was produced (approximately 700 mg per batch). Other values for final rhFI...Continue Reading

References

Jun 1, 1978·The Journal of Clinical Investigation·R G Di ScipioE W Davie
May 20, 1988·Cell·B Furie, B C Furie
Aug 31, 2000·Biologicals : Journal of the International Association of Biological Standardization·A JohnstonE Uren
Mar 22, 2001·Haemophilia : the Official Journal of the World Federation of Hemophilia·P ChowdaryP W Collins
Apr 3, 2001·Trends in Cardiovascular Medicine·D Gailani
Aug 6, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·M BlosteinJ Galipeau

❮ Previous
Next ❯

Citations

Oct 1, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Daniel A RibeiroLeda R Castilho
Jan 8, 2014·Biologicals : Journal of the International Association of Biological Standardization·Jen-Ting ChangShih-Rong Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Absence Epilepsy

Absence epilepsy is a common seizure disorder in children which can produce chronic psychosocial sequelae. Discover the latest research on absence epilepsies here.

© 2021 Meta ULC. All rights reserved