Pilot-scale steam explosion pretreatment with 2-naphthol to overcome high softwood recalcitrance

Biotechnology for Biofuels
Thomas PielhopPhilipp Rudolf von Rohr

Abstract

Steam explosion pretreatment has been examined in many studies for enhancing the enzymatic digestibility of lignocellulosic biomass and is currently the most common pretreatment method in commercial biorefineries. It is however not effective for overcoming the extremely high recalcitrance of softwood to biochemical conversion. Recent fundamental research in small-scale liquid hot water pretreatment has shown, though, that the addition of a carbocation scavenger like 2-naphthol can prevent lignin repolymerization and thus enhance the enzymatic digestibility of softwood cellulose. This work studies the technical application potential of this approach in a larger steam explosion pilot plant for surmounting softwood recalcitrance. The addition of 35.36 g 2-naphthol to the steam explosion pretreatment of 1.5 kg spruce wood chips allowed to considerably enhance the enzymatic cellulose digestibility. Different ways of adding the solid 2-naphthol to steam pretreatment were tested. Mixing with the biomass before pretreatment could enhance digestibility by up to 55% compared to control experiments. Impregnation of the biomass with 2-naphthol was yet more effective. Acetone and ethanol were tested to dissolve 2-naphthol and impregnate the...Continue Reading

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Citations

Mar 5, 2019·Biotechnology for Biofuels·Christoph-Maximilian SeidelMichael H Studer
May 8, 2019·Journal of Agricultural and Food Chemistry·Sebastien Ngwa ObameNicolas Brosse

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BETA
chips

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