Pilot Study Evaluating a Rat Model of Radiation-induced Erectile Dysfunction Using an Image-guided Microirradiator

Urology
Masaki KimuraZeljko Vujaskovic

Abstract

To establish a feasible rat model of radiation-induced erectile dysfunction after targeted prostate irradiation using an image-guided irradiation unit specially designed for small-animal radiation research. The X-RAD 225Cx research platform was used in the present study. We first performed quality assurance testing using a rat cadaver. After confirming dosimetry, 24 age-matched, young, adult, male rats were assigned to sham radiation or radiation to the prostate with doses of 15, 20, or 25 Gy. To confirm appropriate prostate irradiation, physiological erectile function was evaluated using intracavernous pressure (ICP) measurements with cavernous nerve electrical stimulation at 9 weeks after radiotherapy. Each animal was weighed at the time of ICP measurement. In addition, we investigated the cyclic guanosine monophosphate level in the penile cavernosa using a commercial enzyme-linked immunosorbent assay kit. Quality assurance results confirmed the accuracy of the irradiation technique. Dose-dependent decreases in ICP in irradiated rats were observed without major toxicity. No difference in body weight was noted among the experimental groups. Cyclic guanosine monophosphate levels were significantly decreased in the group that re...Continue Reading

References

Mar 22, 2000·International Journal of Radiation Oncology, Biology, Physics·S J DiBiaseS Sutlief
Jun 27, 2001·International Journal of Impotence Research·S L MerlinS B Dion
Sep 16, 2004·Journal of the National Cancer Institute·Arnold L PotoskyLinda C Harlan
Mar 3, 2007·International Journal of Radiation Oncology, Biology, Physics·Gerard J van der WielenLuca Incrocci
Jan 24, 2008·The Prostate·Kenneth J PientaRobert L Vessella
Jul 22, 2008·International Journal of Radiation Oncology, Biology, Physics·John WongTheodore L DeWeese
Mar 5, 2010·International Journal of Radiation Oncology, Biology, Physics·Mack RoachLawrence B Marks
Dec 15, 2010·The Journal of Sexual Medicine·Bridget F KoontzFang-Fang Yin
Jun 18, 2011·The Journal of Sexual Medicine·Masaki KimuraBridget F Koontz

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Citations

Feb 10, 2016·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·G DelponS Chiavassa
Apr 14, 2016·Journal of Oncology Practice·Maria VoznesenskyKarl J Kreder
Oct 10, 2018·The British Journal of Radiology·Matthew C WalbErik J Tryggestad
Jun 23, 2020·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Javed MahmoodZeljko Vujaskovic

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