Pilot study for detection of early changes in tissue associated with heterotopic ossification: moving toward clinical use of Raman spectroscopy

Connective Tissue Research
Mitchell HarrisNicole J Crane

Abstract

Over 60% of combat-wounded patients develop heterotopic ossification (HO). Nearly 33% of them require surgical excision for symptomatic lesions, a procedure that is both fraught with complications and can delay or regress functional rehabilitation. Relative medical contraindications limit widespread use of conventional means of primary prophylaxis, such as nonspecific nonsteroidal anti-inflammatory medications and radiotherapy. Better methods for risk stratification are needed to both mitigate the risk of current means of primary prophylaxis as well as to evaluate novel preventive strategies currently in development. We asked whether Raman spectral changes, measured ex vivo, could be associated with histologic evidence of the earliest signs of HO formation and substance P (SP) expression in tissue biopsies from the wounds of combat casualties. In this pilot study, we compared normal muscle tissue, injured muscle tissue, very early HO lesions (< 16 d post-injury), early HO lesions (> 16 d post-injury) and mature HO lesions. The Raman spectra of these tissues demonstrate clear differences in the Amide I and III spectral regions of HO lesions compared to normal tissue, denoted by changes in the Amide I band center (p < 0.01) and t...Continue Reading

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Citations

Jan 21, 2016·Advanced Healthcare Materials·Neil M EisensteinLiam M Grover
Apr 2, 2017·Hand Clinics·Shailesh AgarwalBenjamin Levi
Sep 30, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Benjamin M WheatleyThomas A Davis
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Jun 1, 2021·Curēus·Margarita-Michaela AmpadiotakiMaria-Eleftheria Evangelopoulos
Aug 8, 2021·Diagnostics·Giuseppe AcriBarbara Testagrossa

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