Pilot Study of 64 CuCl₂ for PET Imaging of Inflammation

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Lei JiangZhen Cheng

Abstract

Copper(II) ion (Cu2+) is the essential element for numerous pathophysiological processes in vivo. Copper transporter 1 (CTR1) is mainly responsible for maintaining Cu2+ accumulation in cells, which has been found to be over-expressed in inflammatory tissues. Therefore, we explored the potential application of 64CuCl₂ for PET imaging of inflammation through targeting CTR1. The animal models of H₂O₂ induced muscle inflammation and lipopolysaccaharide induced lung inflammation were successfully established, then imaged by small animal PET (PET/CT) post-injection of 64CuCl₂, and PET images were quantitatively analyzed. H&E and immunohistochemical (IHC) staining and western blot experiments were performed for evaluating CTR1 levels in the inflammatory and control tissues. Both inflammatory muscle and lungs can be clearly imaged by PET. PET image quantitative analysis revealed that the inflammatory muscle and lungs showed significantly higher 64Cu accumulation than the controls, respectively (p < 0.05). Furthermore, IHC staining and western blot analysis demonstrated that compared with the controls, CTR1 expression was increased in both the inflammatory muscle and lungs, which was consistent with the levels of 64Cu2+ accumulation in ...Continue Reading

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Citations

Feb 16, 2019·Technology in Cancer Research & Treatment·Yeye ZhouYiwei Wu
Jun 11, 2019·Molecular Medicine Reports·Yutao PanZengchun Li
Jun 23, 2020·Annals of Nuclear Medicine·Qiang WangLei Jiang

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