Pilot study of placental tissue collection, processing, and measurement procedures for large scale assessment of placental inflammation

PloS One
Lindsey A SjaardaEnrique F Schisterman

Abstract

Placental dysfunction is related to many pregnancy complications, but collecting placental specimens for investigation in large scale epidemiologic studies is often infeasible. Standard procedures involving immediate collection after birth and snap freezing are often cost prohibitive. We aimed to collect pilot data regarding the feasibility and precision of a simpler approach, the collection of tissue samples following 24 hours of refrigeration of whole placentae at 4°C, as compared to the "gold standard" of snap freezing excised tissue within 40 minutes of delivery for the assessment of inflammatory cytokines. Placentae were collected from 12 women after delivering live-born singleton babies via uncomplicated vaginal delivery. Two placentae were utilized to establish laboratory tissue processing and assay protocols. The other 10 placentae were utilized in a comparison of three tissue collection conditions. Specifically, key inflammatory cytokines were measured in 3 sections, representing three collection conditions. Sections 1 (full thickness) and 2 (excised prior to freezing) were obtained within 40 minutes of delivery and snap frozen in liquid nitrogen, and section 3 (full thickness) was obtained after refrigerating the plac...Continue Reading

References

May 1, 1986·Placenta·B J BaconL D Longo
Nov 21, 2008·BMC Pregnancy and Childbirth·Dharmintra PasupathyGordon C S Smith
Jul 6, 2010·Lancet·Eric A P SteegersRobert Pijnenborg
Oct 27, 2011·Prenatal Diagnosis·N J CowansK Spencer
Oct 26, 2012·Journal of Obstetric, Gynecologic, and Neonatal Nursing : JOGNN·Jane G RyanJoan Rosen Bloch
Dec 3, 2013·Placenta·G J BurtonC W Redman
Mar 5, 2015·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·H StepanA Galindo
Mar 10, 2015·Obstetrics and Gynecology·Robert M SilverEnrique F Schisterman
Nov 5, 2015·European Journal of Cell Biology·Amanda R HighetClaire T Roberts
Oct 11, 2016·Lancet·Marc A RodgerUNKNOWN Low-Molecular-Weight Heparin for Placenta-Mediated Pregnancy Complications Study Group

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Citations

Sep 7, 2021·Rapid Communications in Mass Spectrometry : RCM·Allyson L MellingerTaufika Islam Williams

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Methods Mentioned

BETA
dissection
Protein Extraction
ELISA
Protein

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