Pilot study on the effects of dietary conjugated linoleic acid on tumorigenesis and gene expression in PyMT transgenic mice.

Carcinogenesis
Margaret FlowersPatricia A Thompson

Abstract

Conjugated linoleic acid (CLA) is a class of commercially available fatty acids that have been associated with anticancer properties in rodent models of chemical carcinogenesis. We conducted a pilot study to examine the antitumor effect of dietary CLA in a polyoma virus-middle T antigen (PyMT) mouse model of invasive breast cancer. Virgin 4-week-old PyMT mice were administered a mixed-isomer CLA diet (1% wt/wt) or control AIN-93G diet for 4 weeks (N = 6 and 5, respectively) and tumor burden was assessed at 8 weeks of age. Thoracic mammary glands were prepared as whole mounts with other glands being formalin fixed and paraffin embedded for histology and immunohistochemistry (IHC). Total RNA was prepared for microarray and real-time reverse transcription-polymerase chain reaction analysis. Western blots were performed for protein expression analysis. Tumor incidence was significantly increased in CLA-treated animals compared with controls (P = 0.009) and occurred with extensive lobular-alveolar expansion and loss of mammary adipose tissue. More than 100 genes were downregulated > or = 2-fold in the CLA-treated group compared with controls, including adipose-specific markers, as wells as cytoskeletal and adhesion-related genes. Th...Continue Reading

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Citations

Feb 5, 2016·Journal of Cellular Physiology·William B KinlawNancy B Kuemmerle
Jul 10, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robyn BruenOrina Belton
May 18, 2017·Cardiovascular Research·Barbara Kutryb-ZajacRyszard T Smolenski
Feb 6, 2017·British Journal of Cancer·Mariann Fagernæs HansenPia M Martensen
Jun 23, 2019·Journal of Mammary Gland Biology and Neoplasia·Matthew R Swiatnicki, Eran R Andrechek

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