Pilus chaperones represent a new type of protein-folding catalyst

Nature
Michael VetschRudi Glockshuber

Abstract

Adhesive type 1 pili from uropathogenic Escherichia coli strains have a crucial role during infection by mediating the attachment to and potentially the invasion of host tissue. These filamentous, highly oligomeric protein complexes are assembled by the 'chaperone-usher' pathway, in which the individual pilus subunits fold in the bacterial periplasm and form stoichiometric complexes with a periplasmic chaperone molecule that is essential for pilus assembly. The chaperone subsequently delivers the subunits to an assembly platform (usher) in the outer membrane, which mediates subunit assembly and translocation to the cell surface. Here we show that the periplasmic type 1 pilus chaperone FimC binds non-native pilus subunits and accelerates folding of the subunit FimG by 100-fold. Moreover, we find that the FimC-FimG complex is formed quantitatively and very rapidly when folding of FimG is initiated in the presence of both FimC and the assembly-competent subunit FimF, even though the FimC-FimG complex is thermodynamically less stable than the FimF-FimG complex. FimC thus represents a previously unknown type of protein-folding catalyst, and simultaneously acts as a kinetic trap preventing spontaneous subunit assembly in the periplasm.

References

Mar 19, 1992·Nature·D BakerD A Agard
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M J KuehnS J Hultgren
Jan 1, 1990·Molecular & General Genetics : MGG·P Klemm, G Christiansen
Nov 1, 1989·Journal of Bacteriology·F LindbergS Normark
May 1, 1995·Molecular Microbiology·J Eder, A R Fersht
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K W DodsonS J Hultgren
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C H JonesS J Hultgren
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·D G ThanassiS J Hultgren
Aug 14, 1999·Science·F G SauerG Waksman
Mar 29, 2000·Seminars in Cell & Developmental Biology·V R Agashe, F U Hartl
Apr 8, 2000·Trends in Cell Biology·A R FrandC A Kaiser
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·E T SaulinoS J Hultgren
Oct 24, 2000·Current Opinion in Structural Biology·F G SauerS J Hultgren
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
May 22, 2002·Nature Structural Biology·Cordelia Schiene-FischerGunter Fischer
Aug 16, 2002·Molecular Microbiology·A V ZavialovS D Knight
Sep 25, 2002·Journal of Molecular Biology·Michael VetschRudi Glockshuber
Nov 6, 2002·Journal of Molecular Biology·Erik HahnShirley A Müller

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Citations

Jun 8, 2007·European Biophysics Journal : EBJ·Robert A LugmaierMartin Benoit
Apr 13, 2013·Nature·Sebastian GeibelGabriel Waksman
Jul 10, 2012·Nature Chemical Biology·Maria D CrespoRudi Glockshuber
Oct 13, 2009·Nature Reviews. Microbiology·Gabriel Waksman, Scott J Hultgren
May 26, 2010·The Journal of Biological Chemistry·Sebastian Falk, Irmgard Sinning
Mar 29, 2008·Science·Mireille NishiyamaRudi Glockshuber
Sep 20, 2006·Infection and Immunity·David A ChaltonJeremy H Lakey
Dec 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·James G BannScott J Hultgren
Nov 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jerome S PinknerFredrik Almqvist
Aug 27, 2014·Future Microbiology·David SteadmanHan Remaut
Oct 29, 2014·ELife·Han Remaut, Nir Ben-Tal
Aug 13, 2015·Angewandte Chemie·Birgit HabensteinAdam Lange
Jun 24, 2006·Microbes and Infection·Guido CapitaniMarkus G Grütter
May 16, 2007·Molecular Microbiology·Kris PauwelsPatrick Van Gelder
Jun 13, 2006·EMBO Reports·Michael VetschRudi Glockshuber
Oct 27, 2006·Proteins·Guido CapitaniMarkus G Grütter
Feb 8, 2005·Current Opinion in Microbiology·Andrew L KauScott J Hultgren
Jul 11, 2006·Trends in Biochemical Sciences·Han Remaut, Gabriel Waksman
Dec 19, 2014·Chemical Biology & Drug Design·Riccardo ZambelloniAndrew J Roe
Jan 9, 2013·Journal of Molecular Biology·William J AllenGabriel Waksman
Oct 22, 2013·Biochimica Et Biophysica Acta·Sebastian Geibel, Gabriel Waksman
Dec 17, 2008·Structure·Clothilde ManzanoAndréa Dessen
Jan 28, 2015·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Andreas BuschGabriel Waksman

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