PilVax, a novel Lactococcus lactis-based mucosal vaccine platform, stimulates systemic and mucosal immune responses to Staphylococcus aureus.

Immunology and Cell Biology
Fiona ClowFiona J Radcliff

Abstract

Most pathogens initiate infection via the mucosa, therefore delivery of vaccines directly to the mucosa is likely to be advantageous for stimulating protective immunity at the site of entry. PilVax is a novel mucosal vaccine platform that harnesses Lactococcus lactis bacteria engineered to stably express multiple copies of vaccine peptide antigens within pili, hair-like structures which extend from the cell wall. This strategy elicited systemic and mucosal antibody responses to a model antigen after intranasal immunization, but has not been tested for its capacity to stimulate protective mucosal immunity. A well-characterized linear B-cell epitope, D3(22-33) , from the fibronectin-binding protein A of Staphylococcus aureus was successfully introduced into PilVax and delivered intranasally to mice. Specific antipeptide immunoglobulin (Ig) G and IgA antibodies were detected in the serum and respiratory mucosa of vaccinated mice. Responses to the major pilus backbone protein Spy0128 were also assessed; robust antibody responses to this antigen were generated both systemically and in the respiratory and intestinal mucosa. Mice were challenged intranasally with the mouse-adapted S. aureus JSNZ strain and the S. aureus load quantifie...Continue Reading

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Citations

Feb 13, 2021·International Journal of Molecular Sciences·Sofia O D Duarte, Gabriel A Monteiro
Feb 27, 2021·Frontiers in Microbiology·Masanobu Nakata, Bernd Kreikemeyer
Oct 1, 2020·International Journal of Molecular Sciences·Daniel M MrochenSilva Holtfreter
Jul 20, 2021·Advanced Drug Delivery Reviews·Chao PanHeng-Liang Wang

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