Pin1 promotes transforming growth factor-beta-induced migration and invasion.

The Journal of Biological Chemistry
Isao MatsuuraFang Liu

Abstract

Transforming growth factor-beta (TGF-beta) regulates a wide variety of biological activities. It induces potent growth-inhibitory responses in normal cells but promotes migration and invasion of cancer cells. Smads mediate the TGF-beta responses. TGF-beta binding to the cell surface receptors leads to the phosphorylation of Smad2/3 in their C terminus as well as in the proline-rich linker region. The serine/threonine phosphorylation sites in the linker region are followed by the proline residue. Pin1, a peptidyl-prolyl cis/trans isomerase, recognizes phosphorylated serine/threonine-proline motifs. Here we show that Smad2/3 interacts with Pin1 in a TGF-beta-dependent manner. We further show that the phosphorylated threonine 179-proline motif in the Smad3 linker region is the major binding site for Pin1. Although epidermal growth factor also induces phosphorylation of threonine 179 and other residues in the Smad3 linker region the same as TGF-beta, Pin1 is unable to bind to the epidermal growth factor-stimulated Smad3. Further analysis suggests that phosphorylation of Smad3 in the C terminus is necessary for the interaction with Pin1. Depletion of Pin1 by small hairpin RNA does not significantly affect TGF-beta-induced growth-inh...Continue Reading

References

Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·M B DattoX F Wang
Jan 1, 1993·Breast Cancer Research and Treatment·J Behrens
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·J T PriceE C Kohn
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·A Moustakas, D Kardassis
Jun 17, 1998·Genes & Development·M P de CaesteckerR J Lechleider
Apr 10, 1999·Genes & Development·M KretzschmarJ Massagué
Nov 24, 1999·The Journal of Biological Chemistry·R P NagarajanY Chen
Dec 10, 1999·The Journal of Biological Chemistry·P P HuX F Wang
Dec 22, 1999·The Journal of Biological Chemistry·M E EngelH L Moses
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·N G DenissovaF Liu
Jun 30, 2000·The Journal of Biological Chemistry·T López-RoviraF Ventura
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangR Derynck
Oct 5, 2001·Nature Genetics·R DerynckA Balmain
Jun 1, 2002·Science·Liliana Attisano, Jeffrey L Wrana
Jun 18, 2002·Nature Cell Biology·Oliver H Grimm, J B Gurdon
Aug 24, 2002·The Journal of Biological Chemistry·Masayuki FunabaLawrence S Mathews
Feb 8, 2003·Chemistry & Biology·Takafumi UchidaTony Hunter
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Jun 26, 2003·Cancer Science·Kohei MiyazonoTakeshi Imamura
Jul 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anita B Roberts, Lalage M Wakefield
Sep 6, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Fang Liu
Sep 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Fukiko FurukawaKyoichi Inoue
Jan 1, 2004·Genes & Development·Joan Massague
Apr 28, 2004·The American Journal of Pathology·Lere BaoDa-Gong Wang
May 8, 2004·Trends in Biochemical Sciences·Peter ten Dijke, Caroline S Hill
Sep 7, 2004·The International Journal of Developmental Biology·Lara D M Derycke, Marc E Bracke
Dec 22, 2004·Cell Cycle·Fang Liu, Isao Matsuura

❮ Previous
Next ❯

Citations

Jul 23, 2013·Cytokine & Growth Factor Reviews·Koichi Matsuzaki
May 23, 2012·The Journal of Biological Chemistry·Zhong-Jian ShenJames S Malter
Nov 26, 2011·Antioxidants & Redox Signaling·D Allan ButterfieldRukhsana Sultana
Jun 21, 2011·Genes & Development·Eric AragónMaria J Macias
May 16, 2013·PloS One·Zhong-Jian ShenJames S Malter
Mar 11, 2011·World Journal of Gastroenterology : WJG·Jun-Duo HuJin-Lian Chen
Sep 14, 2012·International Journal of Hepatology·Min CongTatiana Kisseleva
Jun 7, 2011·Cell and Tissue Research·Carl-Henrik Heldin, Aristidis Moustakas
Jul 3, 2015·Biological & Pharmaceutical Bulletin·Garam KimHong Seok Choi
Oct 16, 2015·British Journal of Cancer·Kimio ShinodaMasaru Miyazaki
Mar 22, 2016·Journal of Molecular Biology·Timo EichnerDorothee Kern
May 27, 2015·Breast Cancer Research and Treatment·Virginia G KaklamaniWilliam Gradishar
Jun 5, 2012·Cell Cycle·Elizabeth Tarasewicz, Jacqueline S Jeruss
Feb 20, 2015·Nucleic Acids Research·Marianthi KiparakiChristos Delidakis
Mar 19, 2015·PloS One·Pooja Chandrakant Thacker, Devarajan Karunagaran
Apr 19, 2011·Current Opinion in Pharmacology·Martin TheuerkornCordelia Schiene-Fischer
May 27, 2016·Journal of Cellular Physiology·Rabia IslamHyun-Mo Ryoo
Nov 8, 2014·Clinical and Experimental Pharmacology & Physiology·Muhamad A RostamNarin Osman
Sep 5, 2014·Journal of Cellular Physiology·Won-Joon YoonHyun-Mo Ryoo
Sep 5, 2017·Angewandte Chemie·Sem TamaraAneika C Leney
Dec 31, 2017·Chemistry, an Asian Journal·Ke-Jia WuChung-Hang Leung
May 24, 2012·FEBS Letters·Pinglong XuRik Derynck
Jul 25, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Sunghyeok YeJunseok W Hur
Jun 26, 2014·Neuropathology and Applied Neurobiology·Uwe UeberhamThomas Arendt
Sep 2, 2014·Médecine sciences : M/S·Justine Marsolier, Jonathan B Weitzman
Dec 27, 2016·World Journal of Gastroenterology : WJG·Chi-Wai ChengEric Tse
Aug 31, 2018·Cell Death & Disease·Yang ChenYing-Bin Yang
Nov 22, 2018·Frontiers in Oncology·Yew Mun Lee, Yih-Cherng Liou

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.