Pin1At regulates PIN1 polar localization and root gravitropism

Nature Communications
Wanyan XiYih-Cherng Liou

Abstract

Root gravitropism allows plants to establish root systems and its regulation depends on polar auxin transport mediated by PIN-FORMED (PIN) auxin transporters. PINOID (PID) and PROTEIN PHOSPHATASE 2A (PP2A) act antagonistically on reversible phosphorylation of PINs. This regulates polar PIN distribution and auxin transport. Here we show that a peptidyl-prolyl cis/trans isomerase Pin1At regulates root gravitropism. Downregulation of Pin1At suppresses root agravitropic phenotypes of pp2aa and 35S:PID, while overexpression of Pin1At affects root gravitropic responses and enhances the pp2aa agravitropic phenotype. Pin1At also affects auxin transport and polar localization of PIN1 in stele cells, which is mediated by PID and PP2A. Furthermore, Pin1At catalyses the conformational change of the phosphorylated Ser/Thr-Pro motifs of PIN1. Thus, Pin1At mediates the conformational dynamics of PIN1 and affects PID- and PP2A-mediated regulation of PIN1 polar localization, which correlates with the regulation of root gravitropism.

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Citations

Jun 1, 2016·Journal of Experimental Botany·Laia ArmengotYvon Jaillais
Apr 21, 2017·Frontiers in Plant Science·Yuzhou ZhangGuanghui Xiao
Nov 18, 2019·Plant, Cell & Environment·Benedict C OyigaAgim Ballvora
Oct 23, 2019·BMC Plant Biology·Ling GuanMizhen Zhao
Aug 3, 2019·Development·Michael Sauer, Jürgen Kleine-Vehn
Aug 17, 2019·Frontiers in Plant Science·Marta ZwiewkaTomasz Nodzyński
Mar 7, 2019·Frontiers in Plant Science·Guanghui XiaoYu Zhang
Jan 23, 2021·International Journal of Molecular Sciences·Csaba MáthéTamás Garda
Jul 24, 2020·The Journal of Biological Chemistry·Pengchao HaoMarkus Geisler
Jun 5, 2021·The New Phytologist·Despina SamakovliPolydefkis Hatzopoulos

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Methods Mentioned

BETA
transgenic
nuclear magnetic resonance
NMR
PCR
Fluorescence

Software Mentioned

VENUS
DII
Leica Application Suite Advanced Fluorescence

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