Pinacidil improves contractile function and intracellular calcium handling in isolated cardiac myocytes exposed to simulated cardioplegic arrest

The Annals of Thoracic Surgery
Ru LinQiang Xia

Abstract

We examined the effects of pinacidil on contractile function and intracellular calcium in isolated rat cardiomyocytes exposed to cardioplegic solution. Rat myocytes were incubated at 24 degrees C for 2 hours in cardioplegic solution with or without pinacidil (50 micromol/L), then they were perfused with Krebs-Henseleit solution with a gas phase of 95% O2/5% CO2 at the same temperature. Contraction and intracellular calcium transients were then measured by video tracking and spectrofluorometry. During 20 minutes of perfusion after 2 hours in cardioplegic solution with pinacidil, (1) the recovery of contractile function was significantly increased in terms of both amplitude of contraction (98.30% +/- 9.90% versus 81.00% +/- 11.25%; p < 0.05) and peak velocity of cell shortening (100.90% +/- 13.79% versus 76.89% +/- 18.14%; p < 0.01) when compared with myocytes in cardioplegic solution without pinacidil; (2) the amplitudes of the intracellular calcium transients evoked by electrical stimulation and caffeine (10 mmol/L) increased by 23.31% to approximately 40.72% and 61.73%, respectively, compared with those in cardioplegic solution without pinacidil; and (3) the decay time of the caffeine-induced intracellular calcium transient de...Continue Reading

References

Jul 1, 1992·Trends in Pharmacological Sciences·D Escande, I Cavero
Sep 1, 1995·The Annals of Thoracic Surgery·N M CohenA S Wechsler
Jun 1, 1993·Circulation·W H Barry, J H Bridge
Sep 27, 2000·Basic Research in Cardiology·T Sato, E Marbán
Mar 22, 2002·Pflügers Archiv : European journal of physiology·Chun-Mei CaoYue-Liang Shen

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Citations

Mar 19, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·István BaczkóPeter E Light

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