Pineapple peel carboxymethyl cellulose/polyvinyl alcohol/mesoporous silica SBA-15 hydrogel composites for papain immobilization

Carbohydrate Polymers
Hongjie DaiHuihua Huang

Abstract

Hydrogel composites based on pineapple peel carboxymethyl cellulose, polyvinyl alcohol and mesoporous silica SBA-15 were synthesized by an eco-friendly method of repeated freeze-thaw cycles for the application of papain immobilization. The experiment was optimized to obtain an efficient papain immobilization carrier. Simultaneously the immobilization conditions, including enzyme concentration, pH, crosslinker concentration and cross-linking time were optimized. The immobilized papain had maximum activity at low reaction temperature of 40°C and showed pH-sensitivity by exhibiting a rapid decrease of activity within a narrow range from pH 7.0 to pH 7.5. Compared with the free papain, the immobilized papain revealed enhanced pH, thermal and storage stability. After 2h incubation at 80°C, the immobilized papain retained 56% of its initial activity while the free papain only retained 16%. After 10days of storage, 79% of the initial activity was retained for the immobilized papain while only 27% for the free papain.

Citations

Apr 23, 2020·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Rukiye Yavaşer, A Alev Karagözler
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lian-Hua FuPengbo Wan
Nov 14, 2020·International Journal of Biological Macromolecules·Shalu AggarwalSaiqa Ikram
Apr 18, 2021·International Journal of Biological Macromolecules·Yale Luck NunesJosé C S Dos Santos
Aug 11, 2021·International Journal of Biological Macromolecules·Veymar G Tacias-PascacioRoberto Fernandez-Lafuente
Nov 2, 2019·The Journal of Physical Chemistry. B·Szilárd SáringerIstvan Szilagyi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.