Abstract
Scots pine (Pinus sylvestris) is known to be rich in phenolic compounds, which may have anti-inflammatory properties. The present study investigated the anti-inflammatory effects of a knot extract from P. sylvestris and two stilbenes, pinosylvin and monomethylpinosylvin, isolated from the extract. Inflammation is characterized by increased release of pro-inflammatory and regulatory mediators including nitric oxide (NO) produced by the inducible nitric oxide synthase (iNOS) pathway. The knot extract (EC50 values of 3 and 3 μg/mL) as well as two of its constituents, pinosylvin (EC50 values of 13 and 15 μM) and monomethylpinosylvin (EC50 values of 8 and 12 μM), reduced NO production and iNOS expression in activated macrophages. They also inhibited the production of inflammatory cytokines IL-6 and MCP-1. More importantly, pinosylvin and monomethylpinosylvin exerted a clear anti-inflammatory effect (80% inhibition at the dose of 100 mg/kg) in the standard in vivo model, carrageenan-induced paw inflammation in the mouse, with the effect being comparable to that of a known iNOS inhibitor L-NIL. The results reveal that the Scots pine stilbenes pinosylvin and monomethylpinosylvin are potential anti-inflammatory compounds.
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Mar 10, 2001·Life Sciences·M GentilliJ F Savouret
Aug 15, 2002·Life Sciences·Dong-Im ChoKyeong-Man Kim
Jul 19, 2003·Molecular Pharmacology·Aleksi LahtiEeva Moilanen
Apr 16, 2005·Molecular Nutrition & Food Research·Catalina Alarcón de la Lastra, Isabel Villegas
Aug 17, 2005·Current Drug Targets. Inflammation and Allergy·Riku KorhonenEeva Moilanen
Apr 8, 2006·Physiological Reviews·Stefan W RyterAugustine M K Choi
Jun 20, 2006·Free Radical Biology & Medicine·Gi-Su OhHun-Taeg Chung
Jun 20, 2006·Planta medica·Jongsung LeeDeokhoon Park
May 5, 2010·Nitric Oxide : Biology and Chemistry·Andrea PautzHartmut Kleinert
Oct 16, 2014·Biochimica Et Biophysica Acta·Sameer S Kulkarni, Carles Cantó
Citations
Sep 4, 2015·Basic & Clinical Pharmacology & Toxicology·Lauri J MoilanenEeva Moilanen
Mar 17, 2016·Drug Discovery Today·Milla KibbleTero Aittokallio
Apr 28, 2016·World Journal of Microbiology & Biotechnology·Jing-Long LiangJun-Fei Chen
Oct 16, 2016·Journal of the Science of Food and Agriculture·Kostas IoannidisProkopios Magiatis
Nov 6, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mian ZuGuan-Hua Du
Jan 24, 2020·Journal of Separation Science·Julien GabastonJosep Valls Fonayet
Mar 4, 2020·Foods·Sari MäkinenPirjo H Mattila
Jul 31, 2020·Biomolecules·Paulina PecynaMalgorzata Kucinska
Mar 15, 2020·Sleep·Om Prakash KafleFeng Zhang
Mar 2, 2017·The New Phytologist·Tanja PaaselaTeemu H Teeri
Jan 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mirka LaavolaEeva Moilanen
Jul 8, 2017·Planta·A S Dubrovina, K V Kiselev
May 8, 2020·PloS One·Riitta RyytiEeva Moilanen
Dec 23, 2020·Natural Product Reports·Philippe JeandetSeyed Mohammad Nabavi
Jun 5, 2021·Frontiers in Chemistry·Hong PuYong Huang
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Konsta KivimäkiEeva Moilanen
May 28, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Nora PapDaniel Granato
Nov 22, 2018·Journal of Agricultural and Food Chemistry·Antti PemmariEeva Moilanen
Nov 20, 2021·Fitoterapia·Thinley GyeltshenAlex C Bissember