Piperine inhibits aflatoxin B1-induced cytotoxicity and genotoxicity in V79 Chinese hamster cells genetically engineered to express rat cytochrome P4502B1

Journal of Ethnopharmacology
R K ReenJ Singh

Abstract

We have investigated the potential of piperine for inhibiting the activity of cytochrome P4502B1 and protecting against aflatoxin B1 (AFB1) in V79MZr2B1 (r2B1) cells, i.e. V79 Chinese hamster cells engineered for the expression of rat CYP4502B1. The cells were found to contain high activities of 7-methoxycoumarin demethylase (MOCD). Piperine inhibited MOCD in preparations of r2B1 cells with an IC50 of approximately 10 microM. The cells in culture dealkylated 7-methoxycoumarin (MOC) to 7-OH-coumarin linearly, at least for 12 h, where piperine produced concentration-dependent inhibition with IC50 < 30 microM. The time required for maximal inhibition was approximately 8 h with both 30 and 60 microM concentrations of piperine used. AFB1 at 0.1-20 microM caused a concentration dependent decrease in the amount of DNA and an increase in the formation of micronuclei (MN). The mycotoxin at 10 microM reduced DNA by approximately 30% and increased MN appearance by 20-fold against the background level of 7 MN per 500 nuclei. Piperine at 60 microM completely counteracted cytotoxicity and formation of MN by 10 microM AFB1 and reduced the toxic effects of 20 microM AFB1 by > 50%. The results suggest that: (i) Piperine is a potent inhibitor of...Continue Reading

References

May 1, 1992·Chemical Research in Toxicology·K D RaneyT M Harris
May 1, 1991·Toxicology and Applied Pharmacology·H S RamsdellD L Eaton
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T AoyamaF J Gonzalez
Jun 1, 1990·British Journal of Clinical Pharmacology·D SesardicD S Davies
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T Shimada, F P Guengerich
Jan 1, 1989·Critical Reviews in Toxicology·D W Nebert
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J DoehmerF Oesch
Nov 1, 1994·European Journal of Pharmacology·P PelkonenR O Juvonen
Aug 2, 1993·European Journal of Pharmacology·P SalonpääH Raunio

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Citations

Nov 28, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R El HamssA Muñoz Serrano
Jun 11, 1999·Journal of Ethnopharmacology·T MaliniP Govindarajulu
Feb 23, 2012·Journal of Agricultural and Food Chemistry·Corinna DawidThomas Hofmann
Aug 8, 2002·Kidney International·Hua LiuRadhakrishna Baliga
Nov 8, 2007·Critical Reviews in Food Science and Nutrition·K Srinivasan
Jun 19, 2013·Critical Reviews in Food Science and Nutrition·Masood Sadiq ButtWaqas Ahmed
Oct 3, 2012·TheScientificWorldJournal·Ghanshyam B DudhatraBhavesh N Chauhan
May 4, 2010·Journal of Ethnopharmacology·R Cariño-CortésH Ponce-Monter
Apr 30, 2013·Phytotherapy Research : PTR·Murlidhar Meghwal, T K Goswami
Mar 15, 2003·Drug Metabolism Reviews·Shufeng ZhouJames W Paxton
Nov 4, 2016·Toxins·Verônica da Silva CardosoMaria das Graças Miranda Danelli
Nov 26, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yi LinLei Pan
Feb 24, 2001·Journal of Food Protection·F GalvanoG Galvano
Dec 9, 2016·MedChemComm·R VenkateshwarluS Aravind
Jul 4, 2019·Current Pharmaceutical Design·Katarina SmilkovMustafa Atalay
Oct 27, 2018·Frontiers in Veterinary Science·Begum Yurdakok-DikmenAyhan Filazi
Mar 16, 2021·Toxicon : Official Journal of the International Society on Toxinology·Tingting FanWeibin Ma
Apr 5, 2008·Journal of Agricultural and Food Chemistry·Mendel FriedmanNobuyuki Kozukue

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