Apr 14, 2020

Piplartine attenuates the proliferation of hepatocellular carcinoma cells via regulating hsa_circ_100338 expression

Cancer Medicine
Xiaoli ChengXuetao Yan

Abstract

Researches have pointed that piplartine inhibits the proliferation of hepatocellular carcinoma (HCC) cells, however, the underlying mechanisms has not been well defined. Currently, more and more studies have pointed out that circRNAs can regulate tumor cell proliferation, involve in the tumorigenesis mechanism of various tumors. In this study, we explored whether piplartine may participate in the development of HCC through the regulation of ability of HCC cell proliferation by circRNA. Based on the chip analysis, we selected candidate circRNAs that are highly correlated with HCC. CircRNA expression in OSCC cells treated with piplartine was detected by qRT-PCR. We found that only the expression of hsa_circ_100338 (circ-100338) was observably reduced. The expression characteristics of circ-100338 in HCC cell lines were also verified by qRT-PCR. Subsequently, whether or notcirc-100338 can regulate ZEB1 via competitively binding to miR-141-3p was determined by the RIP assay and dual luciferase reporter gene assay. The effect of the circ-100338/miR-141-3p/ZEB1 axis on the proliferation of HCC cell was tested by EdU and CCK-8 assay. Results showed that circ-100338 expression was observably increased in HCC cell lines. Simultaneously,...Continue Reading

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Mentioned in this Paper

Cell Proliferation
ZEB1 gene
Neoplasms
Protein Expression
Other Anomalies of Pulmonary Artery and Pulmonary Circulation
Detected (Finding)
Piplartine
Binding (Molecular Function)
MIRN141 microRNA, human
Cholecystokinin Measurement

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