Pirfenidone: a novel pharmacological agent that inhibits leiomyoma cell proliferation and collagen production

The Journal of Clinical Endocrinology and Metabolism
B S LeeR A Nowak

Abstract

There are currently no effective, long-term drug therapies for the treatment of leiomyomas. Pirfenidone (Marnac, Inc.) is an antifibrotic agent that is being tested for use in patients with pulmonary fibrosis. Because leiomyomas are characterized also by increased cell proliferation and tissue fibrosis, we examined the effects of pirfenidone on cell proliferation and collagen expression in cultured myometrial and leiomyoma smooth muscle cells. Effects of pirfenidone on proliferation of myometrial and leiomyoma cells were measured using tritiated thymidine incorporation assays and changes in actual cell numbers. Possible cytotoxic effects were examined using lactate dehydrogenase assays and trypan blue exclusion. Effects on collagen type I and type III production were assessed by Northern blotting. Doses of pirfenidone tested were: 0, 0.01, 0.1, 0.3, and 1.0 mg/mL. Serum-stimulated increases in DNA synthesis and cell proliferation by myometrial and leiomyoma cells were significantly inhibited in a dose-dependent manner by pirfenidone. Densitometric analysis of Northern blots showed significantly decreased expression of collagen type I and type III messenger RNAs in both leiomyoma and myometrial cells. Lactate dehydrogenase assay...Continue Reading

Citations

Feb 28, 2002·British Journal of Pharmacology·Stevo MirkovicLindsay Brown
Jan 22, 2011·Obstetrics and Gynecology·Shannon K Laughlin, Elizabeth A Stewart
May 6, 2006·American Journal of Respiratory Cell and Molecular Biology·Atsushi HiranoMitsune Tanimoto
Feb 23, 2011·Therapeutics and Clinical Risk Management·Ye GanRichard H Gomer
Mar 13, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Enrico ConteCarlo Vancheri
Oct 26, 2002·Journal of Hepatology·Antonio Di SarioAntonio Benedetti
Mar 7, 2008·Expert Opinion on Emerging Drugs·Ming-Huei Cheng, Peng-Hui Wang
Jan 23, 2016·Best Practice & Research. Clinical Obstetrics & Gynaecology·Banafsheh N KashaniFelice Petraglia
Nov 11, 2011·Journal de gynécologie, obstétrique et biologie de la reproduction·M KoskasJ Derrien
Jul 31, 2007·Fertility and Sterility·William H Parker
Mar 1, 2006·Obstetrics and Gynecology Clinics of North America·Beth W Rackow, Aydin Arici
Dec 24, 2014·Arthritis Research & Therapy·Marco Rodríguez-CastellanosElizabeth Guevara-Gutiérrez
May 14, 2016·Expert Opinion on Drug Safety·Adam AndersonSteven D Nathan
Oct 21, 2016·Expert Opinion on Drug Safety·Sophie FletcherLuca Richeldi
Mar 8, 2017·Expert Review of Clinical Pharmacology·Peter M George, Athol U Wells
Apr 4, 2017·Lancet·Luca RicheldiMark G Jones
May 14, 2014·Reproductive Sciences·Leo DohertyHugh Taylor
Dec 2, 2017·International Journal of Molecular Sciences·Michał CiebieraGrzegorz Jakiel
May 10, 2001·Clinical Obstetrics and Gynecology·R A Nowak
Oct 31, 2007·Aesthetic Plastic Surgery·Matias GancedoJuan Armendáriz-Borunda
Jun 11, 2019·The Laryngoscope·Takao YamadaYorihisa Orita
Aug 3, 2020·Current Opinion in Obstetrics & Gynecology·Esra CetinMichał Ciebiera
Jul 9, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Fuad S ShihabTakeshi F Andoh
Jun 20, 2003·Multiple Sclerosis : Clinical and Laboratory Research·James D BowenSolomon B Margolin
Feb 9, 2013·The Journal of Clinical Endocrinology and Metabolism·Md Soriful IslamPasquapina Ciarmela
Jan 1, 2010·Expert Review of Endocrinology & Metabolism·Satish P RamachandraRaoKumar Sharma
Jun 30, 2019·International Journal of Pharmaceutics·Rummenigge Oliveira SilvaArmando Silva-Cunha
Jun 11, 2011·Annals of Plastic Surgery·Juan Armendariz-BorundaMaria G Sanchez-Parada
Aug 31, 2014·Fertility and Sterility·Nathalie Chabbert-BuffetPhilippe Bouchard
Apr 6, 2013·Fertility and Sterility·Md Soriful IslamPasquapina Ciarmela
Apr 26, 2006·American Journal of Obstetrics and Gynecology·Phyllis C LeppertJames H Segars
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·R Dale BrownCarlin S Long

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.