Pirfenidone reduces profibrotic responses in human dermal myofibroblasts, in vitro

Laboratory Investigation; a Journal of Technical Methods and Pathology
Caroline L HallKai P Leung

Abstract

Pirfenidone (PFD) is a synthetic small molecule inhibitor with demonstrated anti-inflammatory and antifibrotic properties in vitro and in vivo. The exact mechanism(s) of PFD action remain unclear, due in part to the broad effects of this drug on the complex processes involved in inflammation and fibrosis. While PFD is FDA-approved for the treatment of idiopathic pulmonary fibrosis, the efficacy of this compound for the treatment of dermal fibrosis has not yet been fully characterized. Dermal fibrosis is the pathological formation of excess fibrous connective tissue of the skin, usually the result of traumatic cutaneous injury. Fibroproliferative scarring, caused by delayed wound healing and prolonged inflammation, remains a major clinical concern with considerable morbidity. Despite efforts to identify a therapeutic that targets the fibrotic pathways involved in wound healing to mitigate scar formation, no satisfactory dermal antifibrotic has yet been identified. We aim to better elucidate the antifibrotic mechanism(s) of PFD activity using an in vitro model of dermal fibrosis. Briefly, cultured human dermal fibroblasts were stimulated with TGF-β1 to induce differentiation into profibrotic myofibroblast cells. A dose-dependent ...Continue Reading

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Citations

Mar 21, 2020·Advances in Skin & Wound Care·Gabriel A MecottMauricio M García-Pérez
Jun 13, 2019·Respiratory Research·Jin JinKazuhisa Takahashi
Dec 27, 2018·The Laryngoscope·Emre Türkmen, Yavuz Selim Pata
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Nov 19, 2020·Scientific Reports·Shankar J EvaniKai P Leung
Nov 5, 2019·Biochemical and Biophysical Research Communications·Adrienne R Wells, Kai P Leung
Apr 15, 2021·Advanced Biology·Andrea De PieriKarin Wuertz-Kozak
Sep 2, 2021·Clinical Reviews in Allergy & Immunology·Alain LescoatDinesh Khanna
Nov 13, 2021·Frontiers in Bioengineering and Biotechnology·João Q CoentroDimitrios I Zeugolis

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Methods Mentioned

BETA
electrophoresis
Profiler
PCR
antibody array

Software Mentioned

Excel
CellProfiler
Image Lab
ImageJ
Compass Simple Western
Protein Simple
GraphPad
GraphPad Prism
PromoCell

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